Reveal epigenetic mechanisms in difficult samples with targeted methylation sequencing

There’s more to the story than DNA sequence

The genetic sequence alone does not represent the full picture of gene expression or cellular function. Epigenetic mechanisms, including DNA methylation, can influence gene activity without changing the DNA sequence. DNA methylation is a stable, heritable, covalent modification to DNA, occurring mainly at CpG dinucleotides, but is also found at non-CpG sites. Methylation is associated with normal developmental processes, as well as the changes that are observable during oncogenesis and other pathological processes, such as gene silencing of tumor suppressor or DNA repair genes. Interrogating DNA methylation degree can be challenging in FFPE and liquid biopsy samples, amplifying the need for robust, reliable solutions that work with low-input samples. QIAGEN’s Sample to Insight solutions for epigenetics applications address these challenges to deliver reliable data and powerful insights.

Are you seeing a true variant or a false positive?

Each bisulfite-treated DNA molecule is tagged with a unique molecular index (UMI) before amplification. During analysis, PCR duplicates and false positives are removed to give an accurate methylation profile.

Can you cover every region, even regions with high GC-content?

QIAseq Enrichment Technology uses only one specific primer plus a universal primer for each amplicon, compared to the traditional 2-primer approach.

This means:

• Lower risk of primer dimers and dropouts
• Higher uniformity
• Higher library complexity
• Only a single pool of primers required

Scaling up your targeted panel content? SPE doesn’t require a primer redesign!