References
- Kundert K, et al. Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs. Nat Commun. 2019;10(1):2127.
- Norbert P, et al. Nucleoside-modified mRNA vaccines induce potent T follicular helper and germinal center B cell responses. J Exp Med. 2018; 215(6): 1571–1588.
- Becskei A, Rahaman S. The life and death of RNA across temperatures. Comput Struct Biotechnol J. 2022;20:4325-4336.
- AbouHaidar MG, Ivanov IG. Non-enzymatic RNA hydrolysis promoted by the combined catalytic activity of buffers and magnesium ions. Zeitschrift für Naturforschung C. 1999;54(7-8): 542-548.
- Wacker WE. The biochemistry of magnesium. Ann N Y Acad Sci. 1969;162(2):717-26.
- Reddy KJ, Gilman M. Preparation of bacterial RNA. Curr Protoc Mol Biol. 2001; 4: Unit 4.4.
- Palazzo AF and Lee ES (2015) Non-coding RNA: what is functional and what is junk? Front. Genet. 6:2.
Next
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- Cell lysis methods and sample homogenization types
- Sample lysis techniques for various biological materials
- Considerations for using bead mills in sample preparation
- Effective use of rotor-stator homogenizers for cell and tissue processing
- Notes on mortar and pestle methods for sample homogenization
- Using syringe and needle for cell lysis and sample homogenization
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- How RNA isolation works
- Why RNA isolation and purification are important
- Methods of RNA isolation and RNA extraction procedures
- Comparisons of RNA isolation and RNA extraction methods
- Special considerations for extracting RNA from different sample sources
- Challenges in RNA isolation and how to overcome them
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