REPLI-g Kit specifications
| Kit | Application | Starting material | Kit output | Yield (µg/reaction) |
Reaction time (h) |
Hands-on time (min) |
|---|---|---|---|---|---|---|
| REPLI-g Single Cell Kit |
Single-cell whole genome amplification |
Single cells, 1–1000 cells, tissue, purified gDNA (1–10 ng) |
Whole genome amplified DNA for analysis by cGH array, microarray, qPCR or NGS with the highest sensitivity |
40 µg |
8–16 h |
15 min |
| REPLI-g WTA Single Cell Kit |
Single-cell whole transcriptome amplification |
Single cells (mammalian), 1–1000 cells, purified RNA (10 pg – 100 ng) |
Whole transcriptome amplified cDNA for subsequent analysis by microarray, qPCR or NGS with the highest sensitivity |
20 µg cDNA |
4 h |
20 min |
| REPLI-g Cell WGA & WTA Kit |
Parrallel whole genome and whole transcriptome amplification starting with a few cells |
25–1000 mammalian cells |
Whole genome amplified DNA and whole transcriptome amplified cDNA |
Approx. 20 µg cDNA, approx. 20 µg DNA |
4 h |
20 min |
| REPLI-g Mini Kit | Small-sample whole genome amplification |
10–40 µg |
10–16 h |
15 min |
||
| REPLI-g UltraFast Mini Kit |
Small-sample whole genome amplification, with a focus on short time-to-result |
Whole genome amplified DNA for subsequent analysis by microarray, cGH array, qPCR, Sanger sequencing or NGS |
7–10 µg |
1.5 h |
15 min |
|
| REPLI-g Midi Kit | Small-sample whole genome amplification, with a focus on high yield |
>10 ng gDNA, 0.5 µl blood or cells (600 cells/µl) |
40 µg |
8–16 h |
15 min |
|
| REPLI-g Screening Kit | Small-sample whole genome amplification, with a focus on high sample throughput |
8 µg |
12–16 h |
15 min |
||
| REPLI-g FFPE Kit | Whole genome amplification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue |
FFPE tissue section (1 cm diameter, 10–10 µm thick); 100 ng gDNA from FFPE tissue |
Standard yield: ≤10; high-yield: ≤40 |
Standard yield: 4 h; high yield: 10 h |
40 min |
|
| REPLI-g Mitochondrial DNA Kit |
Whole genome amplification of mitochondrial DNA |
>1 ng purified gDNA | 8 µg |
8 h |
15 min |