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QIAcuity Nanoplate 26k 8-well (10)
目录编号 / ID. 250031
10 QIAcuity Nanoplate 26k 8-well, 11 Nanoplate Seals
登录 要查看您的账户定价。
Product TypeAccessories
QIAcuity Nanoplate
Nanoplate Seals
Nanoplate Tray
Nanoplate Adapter
每孔分区
26k
8.5k
孔数
8
24
QIAcuity Nanoplates 及附件 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
✓ 快速可靠的（再）订购
特点
- 四款针对不同应用需求的纳米微孔板
- 每孔多达 8500 个或 26,000 个反应分区
- SBS 格式
产品详情
QIAcuity Nanoplate 是微流体数字 PCR 孔板，可让您以每孔最多 8500 个或 26,000 个反应分区运行 8 份、24 份或 96 份样本。所有四款纳米微孔板均旨在 QIAcuity 数字 PCR 仪器上运行。
这些纳米微孔板仅供与 QIAcuity Digital PCR System一起使用。使用 QIAgility 在 QIAcuity Nanoplate 中进行自动化液体处理和 PCR 反应体系构建时应使用专用的 QIAcuity Nanoplate 适配器。操作完成后，将该孔板加载到 QIAcuity Digital PCR System 上进行 dPCR 反应。
您想要进一步了解该产品并让我们的 dPCR 专家联系您吗？请在这里登记，我们将很快与您联系。
绩效
这些孔板经过专门设计，专供用于在 QIAcuity 仪器中进行数字 PCR 反应。QIAGEN 提供的纳米微孔板有四种类型，均为边合成边测序 (Sequencing by Synthesis, SBS) 格式，但规格有所不同，可满足不同的应用需求。
| 类型 | 框架颜色 | 规格 | 应用 |
| Nanoplate 26K 8-well | 浅蓝色 |
8 孔 x 大约 26,000 个反应分区 每孔 40 µL dPCR 反应体系 |
罕见突变检测、 液体活检、基因 表达分析、 病原体检测等。 |
| Nanoplate 26K 24-well | 蓝色 |
24 孔 x 大约 26,000 个反应分区 每孔 40 µL dPCR 反应体系 |
罕见突变检测、 液体活检、基因 表达分析、 病原体检测等。 |
| Nanoplate 8.5K 24-well | 白色 |
24 孔 x 大约 85,000 个反应分区 每孔 12 µL dPCR 反应体系 |
拷贝数变异 分析、基因表达 分析、NGS 文库 定量、基因编辑 检测等。 |
| Nanoplate 8.5K 96-well | 灰色 |
96 孔 x 大约 85,000 个反应分区 每孔 12 µL dPCR 反应体系 |
原理
仅需 3 个简单的步骤——移液及加载、运行实验和分析结果，您就能在 2 小时内获得 dPCR 结果。有关纳米微孔板中 dPCR 反应的原理说明，请参见这里。
程序
与 qPCR 实验一样，样本制备包括将预混液、探针和引物转移到一个 8 孔、24 孔或 96 孔纳米微孔板中，随后添加样本该系统将微分化、热循环和成像集成到单一全自动化的仪器上，使用户可在 2 小时内获得结果。您可在 Suite Software 上执行分析，该软件可针对您的靶序列及质量控制（例如阳性样本或无模板对照 [NTC]）给出以每微升拷贝数表示的浓度。该分析还可在同一局域网 (LAN) 上的远程计算机上执行。
应用
QIAcity 纳米微孔板与 QlAcuity Digital PCR System 和 QIAcity PCR 试剂盒的联合使用可让您执行各种数字 PCR 应用，包括：
- 罕见突变检测
- 拷贝数变异分析
- 基因表达分析
- 病原体检测
- 基因分型
- miRNA 研究
- 细胞和基因治疗
- 残留 DNA 定量
- 废水监测
资源
操作软件 (8)
For Version 2.0
For Version 2
Version 4.0
The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.
After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.
Required QIAcuity Software Suite version: Version 1.2 or higher.
The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.
After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.
Required QIAcuity Software Suite version: Version 1.2 or higher.
Version 2.1
Version 2.1
Version 1.2
The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).
The following browsers are supported in the QIAcuity Software Suite:
-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)
The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.
Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.
The new improvements are as follows:
-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting
For Version 1.2
For Version 1.0
应用说明 (9)
The QIAgility instrument is a liquid handler designed for automating PCR setup. For compatibility with the QIAcuity, we developed an adapter to secure up to two nanoplates onto the deck of the QIAgility. Using the QIAgility software, we have optimized a protocol that works for all nanoplate types and QIAcuity applications. Here we report the performance of a front end automated QIAgility dPCR nanoplate setup procedure for use with the QIAcuity dPCR system.
The QIAcuity digital PCR system combined with the QIAcuity One-Step Viral RT-PCR Kit enables precise detection and quantitation of vector-borne viruses in mosquitoes. The results presented in this comparison study showed that digital PCR is a powerful tool for absolute quantitation of low abundant targets and is a more reliable detection method than qPCR. Multiplexing allows detection and quantitation of multiple targets in a single reaction more efficiently by increasing sample throughput at a reduced cost per target.
Digital PCR is a superior method to qPCR for the detection and absolute quantification of low concentration target templates. There are multiple digital PCR systems on the market that differ in numerous aspects including the amount of dead volume, which is the volume that is loaded but not analyzed by the given instrument. While it has been speculated that dead volume could impact the sensitivity of dPCR applications, here we provide data to support the conclusion that the most important factors in determining the relative sensitivity of each system are template addition volume and template analyzed volume. In summary, data provided herein demonstrate that higher template addition volumes can overcome any limitations that dead volume may have on the sensitivity of a dPCR application.
The goal of this work was to compare performance of quantitative PCR (qPCR) and digital PCR (dPCR) in the quantification of gene expression and Wolbachia abundances in Nasonia parasitoid wasps.
This study tested a workflow for quantitation and qualification of AAV samples using a duplex assay on the QIAcuity dPCR instrument targeting both an insert (GFP) and the viral backbone (AAV2-ITR). With very low intra-assay and inter-assay CVs <6.5%, we demonstrate one of the main benefits of dPCR: reproducibility.
Here we report the use of saliva samples in combination with dPCR as a suitable alternative to screen for individuals infected with SARS-CoV-2.
Here we demonstrate how to optimize your assays on a microfluidic nanoplate-based digital PCR system, the QIAcuity, and provide recommendations for a seamless transfer. Moreover, the QIAcuity dPCR workflow is very similar to qPCR.
Here we compared the performance of qPCR and the nanoplate dPCR techniques. The digital PCR method on the QIAcuity significantly improved precision when measuring copy number states and sensitivity of mutation detection through absolute quantification and reduced standard error. This is advantageous in various applications, including copy number variation analysis, small fold-change and rare mutation detection.
Here we provide an integrated rAAV genome titer method using the QIAcuity Digital PCR (dPCR) System with detailed parameters for high assay performance. Using this optimized method for pre-PCR handling of in-process rAAV samples, the results demonstrated that QIAcuity dPCR system generates the same level of accuracy and precision as the current gold standard ddPCR system but with much faster sample-to-result times (2 hours vs 7 hours) and higher overall throughput and scalability.
产品介绍与指南 (1)
Fast. Scalable. Reliable.
用户使用手册 (2)
For use with QIAcuity systems
July 2021
July 2021
Certificates of Analysis (1)
Application Notes (9)
This study tested a workflow for quantitation and qualification of AAV samples using a duplex assay on the QIAcuity dPCR instrument targeting both an insert (GFP) and the viral backbone (AAV2-ITR). With very low intra-assay and inter-assay CVs <6.5%, we demonstrate one of the main benefits of dPCR: reproducibility.
Here we report the use of saliva samples in combination with dPCR as a suitable alternative to screen for individuals infected with SARS-CoV-2.
Digital PCR is a superior method to qPCR for the detection and absolute quantification of low concentration target templates. There are multiple digital PCR systems on the market that differ in numerous aspects including the amount of dead volume, which is the volume that is loaded but not analyzed by the given instrument. While it has been speculated that dead volume could impact the sensitivity of dPCR applications, here we provide data to support the conclusion that the most important factors in determining the relative sensitivity of each system are template addition volume and template analyzed volume. In summary, data provided herein demonstrate that higher template addition volumes can overcome any limitations that dead volume may have on the sensitivity of a dPCR application.
The QIAgility instrument is a liquid handler designed for automating PCR setup. For compatibility with the QIAcuity, we developed an adapter to secure up to two nanoplates onto the deck of the QIAgility. Using the QIAgility software, we have optimized a protocol that works for all nanoplate types and QIAcuity applications. Here we report the performance of a front end automated QIAgility dPCR nanoplate setup procedure for use with the QIAcuity dPCR system.
The QIAcuity digital PCR system combined with the QIAcuity One-Step Viral RT-PCR Kit enables precise detection and quantitation of vector-borne viruses in mosquitoes. The results presented in this comparison study showed that digital PCR is a powerful tool for absolute quantitation of low abundant targets and is a more reliable detection method than qPCR. Multiplexing allows detection and quantitation of multiple targets in a single reaction more efficiently by increasing sample throughput at a reduced cost per target.
The goal of this work was to compare performance of quantitative PCR (qPCR) and digital PCR (dPCR) in the quantification of gene expression and Wolbachia abundances in Nasonia parasitoid wasps.
Here we provide an integrated rAAV genome titer method using the QIAcuity Digital PCR (dPCR) System with detailed parameters for high assay performance. Using this optimized method for pre-PCR handling of in-process rAAV samples, the results demonstrated that QIAcuity dPCR system generates the same level of accuracy and precision as the current gold standard ddPCR system but with much faster sample-to-result times (2 hours vs 7 hours) and higher overall throughput and scalability.
Here we compared the performance of qPCR and the nanoplate dPCR techniques. The digital PCR method on the QIAcuity significantly improved precision when measuring copy number states and sensitivity of mutation detection through absolute quantification and reduced standard error. This is advantageous in various applications, including copy number variation analysis, small fold-change and rare mutation detection.
Here we demonstrate how to optimize your assays on a microfluidic nanoplate-based digital PCR system, the QIAcuity, and provide recommendations for a seamless transfer. Moreover, the QIAcuity dPCR workflow is very similar to qPCR.
Brochures & Guides (1)
Fast. Scalable. Reliable.
Operating Software (14)
Version 1.0
The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.
This new version of the control software includes system stability and performance reliability improvements. We highly recommend to update the control software of your QIAcuity instrument.
Note: The installation requires approximately 90 minutes.
The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.
This new version of the control software includes system stability and performance reliability improvements. We highly recommend to update the control software of your QIAcuity instrument.
Note: The installation requires approximately 90 minutes.
Version 2.0
The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis
Besides system stability and performance reliability improvements, this new version of the control software offers advanced user management and audit trail functionalities. Together with the QIAcuity Software Suite 2.0, the QIAcuity systems now support 21 CFR Part 11 regulations for users working under GMP.
Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.
Note: Upgrading the QIAcuity Control Software to version 2.0 will require upgrading the QIAcuity Software Suite to version 2.0.
Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.0. It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!
The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis
Besides system stability and performance reliability improvements, this new version of the control software offers advanced user management and audit trail functionalities. Together with the QIAcuity Software Suite 2.0, the QIAcuity systems now support 21 CFR Part 11 regulations for users working under GMP.
Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.
Note: Upgrading the QIAcuity Control Software to version 2.0 will require upgrading the QIAcuity Software Suite to version 2.0.
Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.0. It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!
Version 2.1
Beside of improvements and bug fixes, the new features mainly address the GMP requirements by offering additional support for 21 CFR part 11 compliance and get the software ready for future Nanoplate support.
For more information, please refer to the Release Note: QIAcuity Instrument Control Software, Version 2.1.
SHA1 checksum: 0DD41A8DE46D10E81F538ACB71AC184170D39FE0
Version 1.1.3
Version 1.2
The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).
The following browsers are supported in the QIAcuity Software Suite:
-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)
The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.
Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.
The new improvements are as follows:
-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting
Version 4.0
The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.
After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.
Required QIAcuity Software Suite version: Version 1.2 or higher.
The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.
After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.
Required QIAcuity Software Suite version: Version 1.2 or higher.
Version 2.0
The QIAcuity Software Suite 2.0 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).
This new software version now supports 21 CFR Part 11 regulations for users working under GMP. This includes the following:
-Advanced User Management
-Audit Trail
-Signatures
Additional changes compared with the previous software version include the following:
-Usability improvements
-Improved dust detection
-Hyperwell expansion to CNV and GEX analysis
-CSV export functionality of multiple positive partitions for linkage analysis, drop-off assays, etc.
-Raw data export
Important: A direct update of the Software Suite from version 1.1.3 to 2.0.20 is not possible. Please follow the instructions in the User Manual on how to update to version 1.2.18 first before updating the Software Suite to version 2.0.20.
Before you start upgrading to version 1.1.3, please make sure that all plates are imported and visible in the plate overview of the QIAcuity Software Suite.
Caution: Not following the instructions may result in a loss of your previous plate data!
Please contact QIAGEN Technical Services if you are unsure and require technical support.
SHA1 checksum: C60C4EEC349DF1C037796016D32A93EEC19B54A0
Version 2.1
Beside of usability improvements and bug fixes, the new features address GMP requirements by offering additional support for 21 CFR part 11 compliance, copy number variation (CNV) analysis, support of future Nanoplates, and improved 1D scatterplot views.
For more information, please refer to the Release Note: QIAcuity Software Suite, Version 2.1
SHA1 checksum: 6D51E273B26FA7D7E0EDA843CB3DCD74B38FA5B1
For Version 1.0
For Version 1.2
For Version 2.0
For Version 2
Version 2.1
Version 2.1
Instrument User Manuals (2)
For use with QIAcuity systems
July 2021
July 2021
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