在 GeneGlobe 配置
寻找或定制设计合适的靶标特异性检测和组合，以研究您感兴趣的生物靶标。
Microbial DNA qPCR Assay Kit
目录编号 / ID. 330033
One 20 µl tube of Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 tube Microbial DNA-Free Water (1.35 ml), and 1 tube Microbial qPCR Mastermix (1.35 ml)
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Microbial DNA qPCR Assay Kits 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。
在 GeneGlobe 配置
寻找或定制设计合适的靶标特异性检测和组合，以研究您感兴趣的生物靶标。
特点
- qPCR方法进行微生物检测的完整试剂盒，包含所需的引物、探针、对照和试剂
- 包含经实验验证的Microbial DNA qPCR Assay
- 采用5'水解探针进行高度灵敏的特异性检测
产品详情
Microbial DNA qPCR Assay Kits是real-time PCR微生物检测的完整解决方案。Microbial DNA qPCR Assay Kits含有一个Microbial DNA qPCR Assay，后者含有两种PCR引物（每种10 µM）、以及一种5'水解探针（5 µM）用于定量real-time PCR。该试剂盒还含有成功进行qPCR所需的所有试剂和对照，包括Microbial DNA Positive Control、Positive PCR Control、Microbial DNA-Free Water和Microbial qPCR Mastermix。
绩效
低检测阈限（LLOQ）
LLOQ是指在标准曲线线性范围内的最低模板浓度。所有Microbial DNA qPCR Assays中的93%的LLOQ低于100个基因拷贝（参见 The LLOQ for all Microbial DNA qPCR Assays reveals high sensitivity）。该系列产品中的92%微生物检测产品能达到这一LLOQ，95%的毒力因子检测产品和97%的抗生素耐药性基因检测产品能达到这一LLOQ（参见 The LLOQ for microbial identification Microbial DNA qPCR Assays reveals high sensitivity、 The LLOQ for virulence factor genes Microbial DNA qPCR Assays reveals high sensitivity和 The LLOQ for antibiotic resistance genes Microbial DNA qPCR Assays reveals high sensitivity）。特异性
所有Microbial DNA qPCR Assay都经过严格检验，确保能够灵敏检测某一物种或基因（参见 Microbial DNA qPCR Assays are highly specific）。对于能够检测一种以上五种或基因的产品，产品说明页上附有电脑预测可检测靶标的列表。即便样本中存在多物种，该试剂盒仍有极高的检测特异性，此类样本包括粪便、痰和菌斑（参见 Microbial DNA qPCR Assays display high sensitivity even in complex metagenomic samples）。
查看图表
The lower limit of quantification (LLOQ) for all Microbial DNA qPCR Assays reveals high sensitivity.
The lower limit of quantification (LLOQ) for microbial identification Microbial DNA qPCR Assays reveals high sensitivity.
The lower limit of quantification (LLOQ) for virulence factor gene detection Microbial DNA qPCR Assays reveals high sensitivity.
The lower limit of quantification (LLOQ) for antibiotic resistance gene detection Microbial DNA qPCR Assays reveals high sensitivity.
Microbial DNA qPCR Assays are highly specific.
Microbial DNA qPCR Assays display high sensitivity even in complex metagenomic samples.
The lower limit of quantification (LLOQ) for microbial identification Microbial DNA qPCR Assays reveals high sensitivity.
The lower limit of quantification (LLOQ) for virulence factor gene detection Microbial DNA qPCR Assays reveals high sensitivity.
The lower limit of quantification (LLOQ) for antibiotic resistance gene detection Microbial DNA qPCR Assays reveals high sensitivity.
Microbial DNA qPCR Assays are highly specific.
Microbial DNA qPCR Assays display high sensitivity even in complex metagenomic samples.
原理
Microbial DNA qPCR Assays在进行物种检测时，可检测细菌的16S rRNA基因和真菌核糖体rRNA基因序列；采用PCR扩增引物和水解探针，检测毒力因子基因和抗生素耐药性基因。
程序
Microbial DNA qPCR Assay Kit实验流程简单，能够在各种real-time PCR仪上使用。使用相应的QIAamp试剂盒从样本中分离DNA，然后使用与PCR仪相应的Microbial qPCR Mastermix构建PCR反应体系。为每种样本构建4个独立的PCR反应体系，包括Positive PCR Control、No Template Control、Microbial DNA Positive Control以及Microbial DNA qPCR Assay。进行real-time PCR，然后使用在线数据分析软件或Excel模板文件进行数据分析。
应用
Microbial DNA qPCR Assay Kit高度适用于细菌或真菌物种检测，以及微生物的抗生素耐药性基因或毒力因子检测，适用样本包括粪便、痰、阴道拭子、污水等等。
辅助数据和图表
Limit of detection versus lower limit of quantification.
This chart demonstrates the difference between the limit of detection (LOD) and the lower limit of quantification (LLOQ). The LOD is defined as the lowest concentration at which 95% of the positive samples are detected, whereas the LLOQ is the lowest concentration that falls within the linear range of a standard curve. LOD depends upon the precision of the assay, and requires at least 40 replicates for determination of a positive sample. For the Microbial DNA qPCR Assays, LLOQ is sufficient to determine assay sensitivity.
资源
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