QIAsymphony SP를 이용하여 한 번에 96개의 샘플에서 DNA를 자동으로 분리 정제할 수 있습니다
Products
QIAsymphony DNA Kits은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품들은 질병의 진단, 예방, 또는 치료 목적으로 사용할 수 없습니다.
QIAsymphony DNA Midi Kit (96)
Cat. No. / ID. / ID. 931255
For 96 preps of 1000 µl each: includes 2 reagent cartridges and enzyme racks and accessories
QIAsymphony DNA Mini Kit (192)
Cat. No. / ID. / ID. 931236
For 192 preps of 200 µl each: includes 2 reagent cartridges and enzyme racks and accessories
특징
- 다양한 sample에서 DNA정제
- 사용이 쉬운 prefilled reagent cartridges
- 표준화된 처리과정에 의한 높은 재현성
- Sample volume 1 ml까지 가능
제품 세부 정보
QIAsymphony DNA Kits enable automated purification of DNA from a broad range of sample types on the QIAsymphony SP with sample volumes of up to 1 ml. Genomic DNA can be purified from human whole blood, buffy coat, human and animal tissues, formalin-fixed paraffin-embedded tissue (FFPE), cultured cells, and bacterial cultures. The QIAsymphony DNA Mini Kit also enables purification of viral nucleic acids from human whole blood. Optimized protocols provide high yields of pure DNA, and flexible elution volumes allow DNA concentration to be easily optimized for each downstream application. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
성능
Automated purification of DNA on the QIAsymphony SP provides high yields of DNA (see figure " High yields of DNA from human whole blood"). Spectrophotometric analysis showed that the DNA was highly pure (see figure " Highly pure DNA"). DNA gave high-performance results in downstream PCR of a single-copy gene with no inhibition of PCR observed with 1 µl or 10 µl eluate in the amplification reaction (see figures " High-performance results in PCR — A" and " High-performance results in PCR — B").
그림 참조
원리
QIAsymphony DNA Kits combine the speed and efficiency of silica-based purification of genomic DNA with the convenient handling of magnetic particles (see figure " Efficient processing of magnetic particles"). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.
그림 참조
절차
For tissues, cultured cells, and bacteria protocols, manual sample pretreatment is required. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different elution volumes depending on the protocol. The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples are processed in a single run.
| Protocol | Kit | Sample material | Processed volume |
|---|---|---|---|
| DNA Blood 200 | QIAsymphony DNA Mini Kit (192) | Human whole blood | 200 μl |
| DNA Blood 400 | QIAsymphony DNA Midi Kit (96) | Human whole blood | 400 μl |
| DNA Blood 1000 | QIAsymphony DNA Midi Kit (96) | Human whole blood | 1000 μl |
| DNA Buffy Coat 200 | QIAsymphony DNA Mini Kit (192) | Buffy coat | 200 μl |
| DNA Buffy Coat 400 | QIAsymphony DNA Midi Kit (96) | Buffy coat | 400 μl |
| Tissue LC 200 | QIAsymphony DNA Mini Kit (192) | Pretreated sample lysates from tissue, FFPE tissue, cultured cells, and bacterial cultures; for example: heart (25 mg), lung (25 mg), kidney (25 mg), muscle (50 mg), Jurkat cells (2 x 106cells)* | 200 μl |
| Tissue HC 200 | QIAsymphony DNA Mini Kit (192) | Pretreated sample lysates from tissue, cultured cells, and bacterial cultures; for example: spleen (25 mg), liver (25 mg), Jurkat cells (1 x107 cells), bacteria (4 x 109 cells)* | 200 μl |
| Virus Blood 200 | QIAsymphony DNA Mini Kit (192) | Human whole blood | 200 μl |
응용 분야
DNA purified using QIAsymphony DNA Kits is of high quality and is ready for use in downstream applications, such as:
- PCR and real-time PCR
- Genotyping, STR, and SNP analysis
- Sequencing
지원되는 데이터 및 수치
High yields of DNA from human whole blood.
Total DNA was purified from human whole blood (3 donors, 6 replicates each) on the QIAsymphony SP using the QIAsymphony DNA Mini Kit (200 µl) or QIAsymphony DNA Midi Kit (1000 µl). Blood was collected in BD Vacutainer K2E tubes and stored at 5–8°C prior to use. Yield was determined by spectrophotometric analysis.
사양
| 특징 | 사양 |
|---|---|
| applications | PCR, Real-Time PCR, Genotyping |
| sampletype | Blood; Cell culture (only Mini Kit); Tissue (only Mini Kit) |
| elutionvolume | Mini Kit: 50-200 µl; Midi Kit: 100-500 µl |
| sampleamount | Mini Kit: 200 µl blood, up to 50 mg tissue or up to 1 x 10e7 cells; Midi Kit: 400 µl or 1000 µl blood |
| yield | Mini Kit: 4-10 µg from 200 µl blood; Midi Kit: 8-20 µg from 400 µl blood or 15-50 µg from 1000 µl blood |
리소스
Application/Protocol Documents (7)
Safety Data Sheets (1)
Scientific Posters (1)
Labware Files and Documents (1)
Kit Handbooks (2)
Certificates of Analysis (1)