EZ1&2 Virus Mini Kit v2.0

For automated purification of viral nucleic acids from 1–24 samples per run

S_1295_0_LS_QF_EZ1_2_Virus_Mini_kit_v2_0

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EZ1&2 Virus Mini Kit v2.0 (48)

Cat no. / ID.   955134

For 48 preps: Reagent Cartridges (Virus Mini v2.0), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer AVE, carrier RNA
€488.00
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The name of this kit was changed from "EZ1 Virus Mini Kit v2.0" to "EZ1&2 Virus Mini Kit v2.0" to demonstrate that it is compatible with the new EZ2 Connect instruments as well as the EZ1 instruments. No changes were made to the well-established kit technology.
The EZ1&2 Virus Mini Kit v2.0 is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Simultaneous purification of viral DNA and RNA
  • Higher analytical sensitivity, even with low viral titers
  • Highly reproducible results
  • Linear yields from a wide range of viral titers
  • Prefilled, sealed reagent cartridges for easy handling

Product Details

The EZ1&2 Virus Mini Kit v2.0 provides a fully automated procedure for simultaneous purification of viral DNA and RNA from serum, plasma, CSF, urine, respiratory samples, and other cell-free body fluids for sensitive analytical detection in downstream assays. The kit provides optimized binding conditions for more robust and reproducible capture of nucleic acids, and improved wash conditions for higher analytical sensitivity in downstream assays.

The EZ1 DSP Virus Kit is also available for viral nucleic acid purification for in vitro diagnostics (not available in all countries).

The EZ1&2 Virus Mini Kit v2.0 is automated with the EZ2 Connect (1–24 samples per run). This kit can also be used with the EZ1 Advanced XL (1–14 samples per run).

Principle

Automated purification with the EZ1&2 Virus Mini Kit v2.0 requires use of one of the following:

  • the EZ2 Connect
  • the EZ1 Advanced XL and the EZ1 Advanced XL Virus Card v2.0

EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–24 samples (EZ2 Connect) or 1–14 samples (EZ1 Advanced XL) in a single run. Prefilled and sealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases.

Procedure

Serum or plasma samples (100 µl, 200 µl, or 400 µl) are loaded onto the instrument, and viral nucleic acids are purified in one step following the automated EZ1&2 Virus procedure. Viral nucleic acids are eluted in a volume of 150 µl, 120 µl, 90 µl or 60 µl and are ready to use in downstream applications.

The instrument makes viral nucleic acid purification convenient and reliable. The EZ1&2 Virus Mini Kit v2.0 provides prefilled, sealed reagent cartridges that help to prevent human error during setup of the instrument.

Specifications

FeaturesSpecifications
applicationsPCR, real-time RT-PCR
elutionvolume50–200 µl
technologyMagnetic particles
forautomatedprocessingEZ2 Connect instruments or EZ1 Advanced XL
sampleamount100 µl, 200 µl, 400 µl
processingAutomated
formatSample tube
purificationoftotalrnamirnapolyamrnadnaorproteinViral DNA, viral RNA
timeperrunorperprep40 minutes
mainsampletypeSerum, plasma

Resources

Quick-Start Protocols (1)
Brochures & Guides en (1)
Fully automated low- to medium-throughput purification of nucleic acids
Kit Handbooks (1)
EZ1&2 Virus Handbook
PDF (591KB)
EZ1&2 Virus Mini Kit v2.0
For automated, simultaneous purification of viral DNA and RNA, and bacterial DNA, from 1-14 samples of serum, plasma, and CSF, urine, whole blood, and stool, transport media, respiratory samples, and dried swab samples using EZ1 instruments
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)