QIAamp 96 DNA Swab BioRobot Kit

• Rapid purification of high-quality DNA
• No organic extraction or alcohol precipitation
• Consistent, high yields
• Removal of contaminants and inhibitors

The QIAamp 96 DNA Swab BioRobot Kit provides automated DNA purification from up to 192 swabs on the BioRobot Universal System using proven QIAamp silica-membrane technology. Up to 192 samples are processed in less than 2.5 hours. The procedure is optimized for use with air-dried swabs with plastic shafts and cotton or DACRON tips, although other swab types can be used.

Proven QIAamp 96 technology provides well-to-well uniformity in DNA recovery and purity with no cross-contamination between samples (see figure " Cross-contamination test"). The effective removal of enzyme inhibitors ensures reliable performance in downstream applications such as TaqMan®, LightCycler, and iCycler analyses (see figure " Reliable performance").

DNA isolated with the QIAamp 96 DNA Swab BioRobot Kit is ready to use in a wide range of applications in molecular diagnostics and forensic science:

• Genetic disease testing, such as hemochromatosis or Factor V Leiden
• Identity testing, such as HLA typing
• Paternity testing (see figures " Multiplex STR analysis" and " GeneScan analysis")

No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in four efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp technology yields DNA from swabs ready to use in PCR and blotting procedures.

The QIAamp 96 DNA Swab BioRobot Kit simplifies isolation of DNA from swabs with a fast automated 96-well–plate procedure (see flowchart " QIAamp 96 DNA Swab BioRobot procedure"). The procedure is optimized for use with air-dried swabs with plastic shafts and cotton or DACRON tips, although other swab types can be used. The typical yield for the QIAamp 96 DNA Swab BioRobot Kit is 1–2 µg per swab (buccal swabs), with an elution volume of 150 µl.

QIAamp sample preparation technology is fully licensed.

The QIAamp 96 DNA Swab BioRobot Kit provides QIAamp technology in a convenient 96-well format for high-throughput purification needs. The DNA from up to 96 swab samples is purified in under 2 hours. Sample types include:

• Buccal swabs
• Pharyngeal swabs
• Eye swabs