QIAamp DSP Virus Kit

For purification of viral nucleic acids from human plasma and serum for in vitro diagnostic use

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QIAamp DSP Virus Kit

Cat no. / ID.   60704

For 50 preps: QIAamp MinElute Columns, buffers, reagents, tubes, column extenders, VacConnectors
$495.00
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The QIAamp DSP Virus Kit is intended for in vitro diagnostic use.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Universal purification system compatible with other IVD products
  • High-quality viral nucleic acids
  • Concentration with 20 µl or 60 µl eluates
  • Sample volumes of 500 µl
  • Rapid purification and minimal risk of cross-contamination

Product Details

The QIAamp DSP Virus Kit uses well-established and convenient QIAamp technology for simultaneous purification of viral DNA and RNA for in vitro diagnostic use.

Performance

Viral nucleic acids purified using the QIAamp DSP Virus Kit are ready to use in sensitive downstream applications, such as those based on enzymatic amplification, including PCR and RT-PCR.

Principle

The QIAamp DSP Virus Kit uses well-established and convenient QIAamp technology for simultaneous purification of viral DNA and RNA. The QIAamp silica-based membrane binds nucleic acids in the lysed sample, while the rest of the lysate is rapidly removed by vacuum pressure. The bound nucleic acids are efficiently washed to remove contaminants and then eluted in a volume of 20 µl or 60 µl.

Procedure

The QIAamp DSP Virus Kit Procedure includes the following steps: lyse, bind, wash (3x), dry spin, and elute. Viral nucleic acids are purified from plasma or serum using a vacuum manifold, such as the QIAvac 24 Plus (see flowchart, " Procedure").
See figures

Applications

Viral nucleic acids can be purified from plasma or serum samples. Samples can contain the anticoagulants citrate or EDTA, and can be either fresh, lyophilized, or frozen (provided they were not thawed and refrozen).

The QIAamp DSP Virus Kit provides purification of nucleic acids from a wide range of viruses. The kit is compatible with a wide range of upstream sample collection systems and downstream applications, and therefore can be easily integrated into diagnostic workflows.

Supporting data and figures

Specifications

FeaturesSpecifications
applicationsDownstream detection procedures in molecular diagnostics, such as PCR
elutionvolume20 µl, 60 µl
mainsampletypeSerum, plasma
cefdaivdcompatibleCE/IVD
formatSample tubes
processingManual (vacuum)
purificationoftotalrnamirnapolyamrnadnaorproteinViral DNA, viral RNA
sampleamount500 µl
technologySilica technology
yieldVaries

Resources

Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Brochures & Guides (1)
Certificates of Analysis (1)

Publications

Occurrence of hepatitis A virus genotype III in Germany requires the adaptation of commercially available diagnostic test systems.
Heitmann A; Laue T; Schottstedt V; Dotzauer A; Pichl L;
Transfusion; 2005; 45 (7):1097-105 2005 Jul PMID:15987353