Products
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
QIAamp DSP Circulating NA Kit
Cat. No. / ID. / ID. 61504
For 50 preps: includes QIAamp Mini Columns, Buffers, Carrier RNA, QIAGEN Proteinase K, and Tubes.
Rotor-Gene Q 5plex HRM Platform
Cat. No. / ID. / ID. 9001580
Real-time PCR cycler and High Resolution Melt analyzer with 5 channels (green, yellow, orange, red, crimson) plus HRM channel, laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training not included
QIAamp DNA FFPE Tissue Kit (50)
Cat. No. / ID. / ID. 56404
For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Buffers, Collection Tubes (2 ml)
Use our next-generation QIAamp DNA FFPE Advanced Kits for improved performance.
QIAamp Circulating Nucleic Acid Kit (50)
Cat. No. / ID. / ID. 55114
For 50 preps: QIAamp Mini Columns, Tube Extenders (20 ml), QIAGEN Proteinase K, Carrier RNA, Buffers, VacConnectors, and Collection Tubes (1.5 ml and 2 ml)
Features
- Reliable detection of relevant mutations in the PIK3CA gene
- High sensitivity and specificity
- Results in less than two working days
- Optimized reagents and reaction mixes
- Automated data analysis using Rotor-Gene AssayManager v2.1 software
Product Details
The PIK3CA RGQ PCR Kit is a real-time qualitative PCR test for the detection of 11 mutations in the phosphatidylinositol 3-kinase catalytic subunit alpha (PIK3CA) gene using a sample of DNA extracted from either formalin-fixed, paraffin-embedded (FFPE) breast tumor tissue or K2EDTA anticoagulated blood plasma, taken from a patient with breast cancer.
Principle
The PIK3CA RGQ PCR Kit is comprised of six reaction mixes; one control reaction targeting exon 15 and five mutation-specific reactions utilized to detect 11 mutations in exons 7, 9 and 20 of the PIK3CA gene (Exon 7: C420R; Exon 9: E542K; E545A, E545D [1635G>T only], E545G, E545K, Q546E, Q546R; and Exon 20: H1047L, H1047R, H1047Y). Allele-specific technology allows accurate and highly reproducible detection of mutations; DNA is selectively amplified using ARMS primers, probes and PCR clamps, with sensitive signal detection using the Rotor-Gene Q 5plex HRM instrument. Data analysis is performed automatically. Outputs for each PCR are displayed individually, with "Amplification Detected" displayed when a specific PIK3CA mutation is detected.
Procedure
The simple and straightforward testing workflow begins with manual DNA extraction from either FFPE breast tumor tissue (using the QIAamp DSP DNA FFPE Tissue Kit or QIAamp DNA FFPE Tissue Kit) or from K2EDTA anticoagulated plasma (using the QIAamp DSP Circulating Nucleic Acid Kit or QIAamp Circulating Nucleic Acid Kit), followed by sensitive real-time PCR on the Rotor-Gene Q 5plex HRM instrument. Rotor-Gene AssayManager software rapidly and accurately reports results, informing the system operator if one or more of the 11 mutations detected by the kit are present in each sample. The kit is a qualitative assay that can yield results in less than two working days.
Applications
The PIK3CA RGQ PCR Kit enables qualitative detection of 11 mutations in the PIK3CA gene
Resources
Brochures & Guides en (5)
Brochures & Guides (2)
Quick-Start Protocols (1)
Supplementary Protocols (6)
Kit Handbooks (8)
Scientific Posters (3)
Instrument Technical Documents (3)
Analysis Software (1)
Application/Protocol Documents for User-Validated Use (2)
Safety Data Sheets (2)
Instrument User Manuals (5)
Operating Software (1)
Technical Information (3)
Validation Reports (1)
Application Notes (1)
Application/Protocol Documents (1)
Additional Resources (1)
Certificates of Analysis (1)