RNeasy MinElute Cleanup Kit – RNA Cleanup and Concentration

• RNase, nucleotide 제거 외에 labeling 같은 효소 반응으로 부터의 효율적인 정제
• 민감도 높은 실험을 위해 10ul로 RNA를 농축할 수 있음
• 다른 방법으로 추출된 RNA 정제 — e.g., crude preps from organic extraction methods
• 15분안에 양질의 RNA 추출 — 침전 과정, vacuum 과정, 원심 분리 과정 없음

The RNeasy MinElute Cleanup Kit enables cleanup and concentration of RNA from enzymatic reactions or other samples using specialized RNeasy MinElute spin columns based on silica-membrane technology. The kit can also be used to desalt RNA samples. Up to 45 μg RNA can be purified in a volume as low as 10 μl. Purification can be fully automated on the QIAcube.

The RNeasy MinElute Cleanup Kit is designed to purify and concentrate RNA from enzymatic reactions (e.g., labeling, in vitro transcription), RNA isolated by alcohol-precipitation and organic-extraction methods, as well as to desalt RNA samples, and concentrate RNA prepared by silica-membrane procedures (e.g., PAXgene Blood RNA preps). RNeasy technology simplifies total RNA isolation by combining guanidine-isothiocyanate lysis with silica-membrane purification.

Guanidine-isothiocyanate–containing lysis buffer and ethanol are added to the sample to create conditions that promote selective binding of RNA to the RNeasy MinElute membrane. The sample is then applied to the RNeasy MinElute spin column. RNA binds to the silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in water.

Enzymatic reactions or crude RNA preps are simultaneously cleaned up and concentrated in less than 15 minutes. In comparison, time-consuming concentration and cleanup by alcohol precipitation can result in loss of RNA, especially from small samples. The RNeasy MinElute procedure is faster than vacuum centrifugation, which only concentrates the sample without removing salts and other impurities. The unique design of RNeasy MinElute spin columns enables concentration of purified RNA to as little as 10 µl for downstream applications such as microarray analysis and real-time RT-PCR. Purification can be fully automated on the QIAcube.

RNA purified with RNeasy MinElute technology is high-quality and ideal for use in all applications including:

• Northern, dot, and slot blotting
• End-point RT-PCR
• Quantitative, real-time RT-PCR
• Array analysis
• Poly A+ RNA selection