RT2 SYBR® Green qPCR Mastermixes
用于real-time PCR分析
Products
RT2 SYBR® Green qPCR Mastermixes 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。
RT² SYBR Green Fluor qPCR Mastermix (24)
Cat. No. / ID. / ID. 330513
Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x10 µl reactions
RT² SYBR Green qPCR Mastermix (12)
Cat. No. / ID. / ID. 330502
Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions
RT² SYBR Green ROX qPCR Mastermix (24)
Cat. No. / ID. / ID. 330523
Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x 10 µl reactions
RT² SYBR Green qPCR Mastermix (2)
Cat. No. / ID. / ID. 330500
Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Arrays or 200 x 25 µl reactions or 400 x 10 µl reactions
RT² SYBR Green ROX qPCR Mastermix (25 ml)
Cat. No. / ID. / ID. 330529
Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactions or 5000 x 10 µl reactions
RT² SYBR Green Fluor qPCR Mastermix (12)
Cat. No. / ID. / ID. 330512
Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions
RT² SYBR Green qPCR Mastermix (24)
Cat. No. / ID. / ID. 330503
Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x 10 µl reactions
RT² SYBR Green ROX qPCR Mastermix (6)
Cat. No. / ID. / ID. 330524
Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Array or 600 x 25 µl reactions or 1500 x 10 µl reactions
RT² SYBR Green Fluor qPCR Mastermix (2)
Cat. No. / ID. / ID. 330510
Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactions or 400 x 10 µl reactions
RT² SYBR Green qPCR Mastermix (25 ml)
Cat. No. / ID. / ID. 330509
Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactions or 5000 x 10 µl reactions
RT² SYBR Green ROX qPCR Mastermix (8)
Cat. No. / ID. / ID. 330521
Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions
RT² SYBR Green qPCR Mastermix (8)
Cat. No. / ID. / ID. 330501
Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions
RT² SYBR Green Fluor qPCR Mastermix (25 ml)
Cat. No. / ID. / ID. 330519
Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactions or 5000 x 10 µl reactions
RT² SYBR Green Fluor qPCR Mastermix (6)
Cat. No. / ID. / ID. 330514
Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Array or 600 x 25 µl reactions or 1500 x 10 µl reactions
RT² SYBR Green ROX qPCR Mastermix (12)
Cat. No. / ID. / ID. 330522
Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions
RT² SYBR Green Fluor qPCR Mastermix (8)
Cat. No. / ID. / ID. 330511
Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions
RT² SYBR Green qPCR Mastermix (6)
Cat. No. / ID. / ID. 330504
Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Arrays or 600 x 25 µl reactions or 1500 x 10 µl reactions
RT² SYBR Green ROX qPCR Mastermix (2)
Cat. No. / ID. / ID. 330520
Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactions or 400 x 10 µl reactions
特点
- 特异性扩增，引物二聚体较少
- 一体化混合物，适用于特定仪器
- 可选含有ROX、荧光素或不含染料的规格
产品详情
RT2 SYBR Green qPCR Mastermixes非常适合使用SYBR Green进行real-time PCR分析，可选含有或不含参比染料的规格。RT2 SYBR Green qPCR Mastermixes应与RT2 qPCR Primer Assays和RT2 Profiler PCR Arrays配合使用。
绩效
RT² SYBR Green qPCR Mastermixes包含所有优化的试剂和缓冲液，可在各种real-time PCR分析仪上配合RT2 qPCR Primer Assays和RT2 Profiler PCR Arrays进行SYBR Green real-time PCR分析。
RT² SYBR Green qPCR Mastermixes进行的高性能real-time PCR具有较高的扩增效率（参见" High amplification efficiency over a wide dynamic range"）和高灵敏度及特异性（参见" Tighter control of polymerase activity yields greater specificity"）。
查看图表
原理
RT2 SYBR Green qPCR Mastermixes包含real-time PCR缓冲液、高性能的HotStart DNA Taq聚合酶、核苷酸和SYBR Green染料。有些预混液包含荧光素或ROX染料，用于优化光学仪器。化学修饰并严格控制的HotStart酶可避免扩增引物二聚体和其他非特异性产物，提供准确的SYBR Green结果。
不含ROX或荧光素的预混液
RT2 SYBR Green qPCR Mastermix适合在不需要参比染料的仪器上进行SYBR Green real-time PCR应用，仪器包括Bio-Rad models CFX96、CFX384；Bio-Rad/MJ Research Chromo4、DNA Engine Opticon、DNA Engine Opticon 2；Roche LightCycler 480（96孔和384孔）；无ROX滤过装置的Eppendorf Mastercycler ep realplex和Cepheid SmartCycler。
含有荧光素的预混液
RT2 SYBR Green qPCR Mastermix适合在需要荧光素作为参比染料的仪器上进行SYBR Green real-time PCR应用，仪器包括Bio-Rad models iCycler、iQ5、MyiQ和MyiQ2。
含有ROX的预混液
RT2 SYBR Green qPCR Mastermix适合在需要ROX作为参比染料的仪器上进行SYBR Green real-time PCR应用，仪器包括QIAGEN的Rotor-Gene Q实时荧光定量PCR分析仪；Applied Biosystems models 5700、7000、7300、7500（标准和快速）、7700、7900HT（标准和快速的96-well block，384-well block）、StepOnePlus和ViiA 7（标准和快速的96-well block，384-well block）；含有或无ROX滤过装置的Eppendorf Mastercycler ep realplex；Stratagene models Mx3000P、Mx3005P、Mx4000以及Takara TP-800。
应用
RT2 SYBR Green qPCR Mastermixes适合real-time PCR分析，特别是RT2 qPCR Primer Assays和RT2 Profiler PCR Arrays。
辅助数据和图表
High amplification efficiency over a wide dynamic range.
High amplification efficiency over a wide dynamic range.
资源
安全数据表 (1)
应用说明 (1)
仪器技术参数 (1)
试剂盒操作手册 (1)
Certificates of Analysis (1)