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Proteinase K (5 ml)
Cat no. / ID. 158146
5 ml Puregene Proteinase K
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EnzymeSolution
Enzyme
Solution
Type
Proteinase K
RNase A
Volume
5 ml
650 µl
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Archive-quality DNA for long-term storage
- Reproducible DNA purification
- Convenient, scalable purification procedure
Product Details
Puregene Accessories used in combination with Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows large-volume samples to be purified for use as long-term references.
To improve availability address demand more efficiently we implemented an in-house manufacturing solution. This leads to product name and catalogue number changes . This Change will not reflect on the quality and performance of the products. The legacy products will be discontinued on 28.02.2022 and be available only until stocks last.
Please use the table below in the Performance section to find the product you need.
Performance
| NEW product name | NEW cat.no. | Old product name | Old cat. no. |
| 158103 | Puregene RBC Lysis Solution (450 ml) | 158902 | RBC Lysis Solution(450 ml) |
| 158106 | Puregene RBC Lysis Solution (1000 ml) | 158904 | RBC Lysis Solution (1000 ml) |
| 158113 | Puregene Cell Lysis Solution (125 ml) | 158906 | Cell Lysis Solution (125 ml) |
| 158116 | Puregene Cell Lysis Solution (1000 ml) | 158908 | Cell Lysis Solution (1000 ml) |
| 158123 | Puregene Protein Precipitation Sol. (50 ml) | 158910 | Protein Precipitation Solution (50 ml) |
| 158126 | Puregene Protein Precipitation Solution (350 ml) | 158912 | Protein Precipitation Solution (350 ml) |
| 158133 | Puregene DNA Hydration Solution (100 ml) | 158914 | DNA Hydration Solution (100 ml) |
| 158136 | Puregene DNA Hydration Solution (500 ml) | 158916 | DNA Hydration Solution (500 ml) |
| 158143 | Proteinase K (650 µl) | 158918 | Puregene Proteinase K (650 µl) |
| 158146 | Proteinase K (5 ml) | 158920 | Puregene Proteinase K (5 ml) |
| 158153 | RNase A Solution (650 µl) | 158922 | RNase A Solution (650 µl) |
| 158156 | RNase A Solution (5 ml) | 158924 | RNase A Solution (5 ml) |
| 158183 | Glycogen Solution (500 µl) | 158930 | Glycogen Solution (500 µl) |
| 158186 | Glycogen Solution (5 ml) | 949002 | Autopure Glycogen Solution (5 ml) |
Principle
Using Puregene Kits, cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in hydration solution (1 mM EDTA, 10 mM Tris·Cl pH 7.5). Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C, or –80°C.
Procedure
Archive-quality DNA
Purity of DNA has a significant effect on the accuracy of results obtained in downstream applications. Sensitive downstream applications, such as PCR, demand the use of DNA of the highest quality and molecular weight for reliable results. Puregene Kits removes contaminants and enzyme inhibitors enabling purification of highly stable DNA well-suited for archiving. Purified DNA is of high quality demonstrated by molecular weights of 100–200 kb.
Applications
DNA purified using Puregene Accessories and Puregene Kits is highly stable and suited for use in a wide range of applications, such as:
- DNA archiving
- PCR
- SNP analysis
- Southern blotting
- NGS
- Long-read sequencing
Resources
Certificates of Analysis (1)
Publications
Methylation variation at IGF2 differentially methylated regions and maternal folic acid use before and during pregnancy.
Epigenetics; 2011; 6 (7):928-36 2011 Jul 1 PMID:21636975