QIAsymphony SP for FFPE sample preparation

For automated, high throughput DNA prep from FFPE tissue samples

Products

In the context of the GeneReader NGS System, the QIAsymphony SP, QIAsymphony DPS DNA Mini Kit and QIAsymphony GeneRead FFPE Treatment Kits are intended for Research Use Only. Not for use in diagnostic procedures.
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QIAsymphony GeneRead DNA FFPE Treatment Kit  (192)

Cat. No. / ID. / ID.   185306

For uracil-N-glycosylase treatment of FFPE tissue specimens using the QIAsymphony SP Tissue_LC_200_V7_DSP protocol
€577.00
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QIAsymphony SP

Cat. No. / ID. / ID.   9001297

QIAsymphony sample prep module: includes 1-year warranty on parts and labor
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QIAsymphony DSP DNA Mini Kit (192)

Cat. No. / ID. / ID.   937236

For 192 preps of 200 µl each: Includes 2 reagent cartridges and enzyme racks and accessories.
€1,106.00
The QIAsymphony DSP DNA Mini Kit (192) is intended for in vitro diagnostic use.

Features

  • Rapid purification of high-quality, ready-to-use DNA for use in research applications
  • Enzymatic removal of cytosine deamination artifacts for FFPE samples
  • Automation on the QIAsymphony Sample Preparation
  • Outstanding results in DNA sequencing applications

Product Details

With the QIAsymphony DSP DNA Mini Kit, in combination with the QIAsymphony GeneRead DNA FFPE Treatment Kit and QIAsymphony SP, it is possible to fully automate DNA extraction from up to 96 FFPE samples in parallel. Enzymatic removal of cytosine deamination artifacts from FFPE samples in combination with optimized protocols provide high yields of pure DNA, suitable for downstream sequencing applications in the GeneReader NGS System workflow. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

Principle

The QIAsymphony DSP DNA mini kit in combination with the QIAsymphony GeneRead DNA FFPE Treatment Kit can be used to purify DNA from FFPE tissues. This combines the speed and efficiency of silica-based purification of DNA with the convenient handling of magnetic particles (see figure " Schematic of the QIAsymphony SP principle"). Innovative, ready-to-run reagent cartridges are prefilled with all reagents required for the purification procedure and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves you valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.
See figures

Procedure

The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute (see figure " QIAsymphony DSP DNA procedure"). Optimized protocols provide high quality nucleic acids with efficient removal of proteins, nucleases, and other impurities. Nucleic acids purified with QIAsymphony GeneRead DNA FFPE Treatment Kit and QIAsymphony DSP DNA Mini Kit is ready for direct use in downstream library preparation and sequencing applications on the GeneReader NGS System. The user can choose between different elution volumes.
See figures

Applications

Designed for use in the GeneReader NGS System workflow.

Supporting data and figures

Service Plans

QIAsymphony SP, Preventive Subscription

Cat. No. / ID. / ID.   9243545A

One on-site Preventive Maintenance or Inspection Service visit for the QIAsymphony SP, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.
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Available on demand

Resources

Application/Protocol Documents (27)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
Supporting Software and Tools (3)
For QIAsymphony software version 5.0.4
Work List Tool
SOFTWARE (1MB)
For QIAsymphony Software version 5.0
Language Package – German
SOFTWARE (830KB)
German language package for QIAsymphony software version 5.0.4
Protocol Files (8)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
Instrument User Manuals (4)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
Application/Protocol Documents for IVD Use (7)
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Instrument Technical Documents (10)
QIAsymphony Cabinet SP/AS documents
Differences between QIAsymphony software versions
For use with QIAsymphony SP/AS instruments (software version 5.0)
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony Cabinet SP/AS document
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
Software document for QIAsymphony software version 4.0.1
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
Brochures & Guides en (3)
Advanced sample collection, automation and STR solutions for kinship testing
Labware Files and Documents (2)
Labware package SOW-516-0-08 for use with the QIAsymphony SP/AS instruments (software version 5.0)
Performance Data (1)
Labware Documents (1)
For use with software version 5.0 or higher
Certificates of Analysis (1)