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EpiTect PCR Control DNA Set (100)
Cat no. / ID. 59695
Human control DNA set (containing both bisulfite converted methylated and unmethylated DNA and unconverted unmethylated DNA) for 100 control PCRs
EpiTect Control DNA and Control DNA Set are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Convenient, ready-to-use, quality-controlled DNA
- Bisulfite-converted DNA for control experiments
- Suitable for all methylation analyses
Product Details
EpiTect Control DNAs are ready-to-use, completely methylated or completely unmethylated bisulfite converted DNAs, and untreated, unmethylated genomic DNA, for standardized and reliable control reactions for methylation analysis. The methylated, bisulfite converted DNA as well as unmethylated, bisulfite converted and unconverted DNA are stored in EB Buffer (10 mM Tris Cl) in a ready-to-use solution.
Performance
EpiTect Control DNAs are highly suited for use as quantification standards for probe-based real-time PCR methylation analysis (see figure " Real-time PCR standards") and high-resolution melting (HRM) methylation analysis (see figure " HRM quantification standards").
See figures
Principle
The EpiTect Control DNA Set contains all control DNAs needed for standardized and reliable methylation control reactions in a ready-to-use kit format . In methylation-specific PCR, for example, control reactions must be carried out to ensure that PCR probes and primers bind specifically to methylated or unmethylated DNA (see figure Use of EpiTect Control DNA in PCR for methylation analysis). These reactions require various concentrations of completely bisulfite converted control DNA that is fully methylated or fully unmethylated (see figures EpiTect Control DNA Pyrogram and " EpiTYPER MALDI-TOF analysis of WBA DNA"). Additionally, mixtures of these control DNAs can serve as quantification standards when determining degree of methylation in HRM and probe-based methylation experiments.
See figures
Procedure
Standardized workflows in epigenetics
Accessing epigenetic information is of prime importance for many areas of biological and medical research — particularly oncology, but also stem cell research and developmental biology. However, the analysis of changes in DNA methylation is challenging, due to the lack of standardized methods for providing reproducible data particularly from limited sample material. With its newly introduced EpiTect solutions, QIAGEN makes available standardized, pre-analytical and analytical solutions from DNA sample collection, stabilization and purification, to bisulfite conversion and real-time or end-point PCR methylation analysis or sequencing (see figure " Standardized workflows in epigenetics").
See figures
Applications
EpiTect Control DNAs are suitable for use in control reactions for all types of methylation analyses, including:
- Evaluating primer specificity for MSP
- Quantification standard for HRM and MethyLight PCR
- Evaluating primer and probe specificity for MethyLight PCR
- Checking efficiency of bisulfite conversion reactions
Supporting data and figures
Real-time PCR standards.
EpiTect Methylated Control DNA and EpiTect Unmethylated Control DNA (both pre-bisulfite converted) were mixed to give 100%, 90%, 50%, 10%, and 0% methylated DNA. EpiTect MethyLight Assays for the human PITX2 gene were run in triplicate, using 10 ng of each DNA sample. The results show that EpiTect Control DNA can be used as a methylation standard for the quantification of unknown DNA samples.
Specifications
| Features | Specifications |
|---|---|
| applications | End point Methylation Specific PCR (MSP), real-time methylation specific PCR |
| numberofreactions | for 1000 control PCRs |
| concentration | 10 ng/µl (1 µg in total) excepted the unmethylated control DNA: 50 ng/µl (10 µg in total) |
Resources
Kit Handbooks (5)
Safety Data Sheets (1)
Certificates of Analysis (1)