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FlexiGene DNA Kit (250)
Cat no. / ID. 51206
For isolation of genomic DNA from whole blood, buffy coat, and cultured cells in a single tube with fast kit preparation
The FlexiGene DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Easy handling, no risk of sample mix-up
- Scalable purification from 0.1–20 ml whole blood
- Direct purification from whole blood, buffy coat, and cultured cells
- Fast procedure
- No organic extraction
Product Details
The FlexiGene system uses simple centrifugation to isolate highly pure DNA from whole blood, buffy coat, and cultured cells. The FlexiGene DNA Kit contains buffers and QIAGEN Protease for direct, simple purification in a single tube. For automated solutions using FlexiGene reagents, contact Autogen, Inc.
Performance
DNA purified using the FlexiGene system is sized up to 150 kb (see figure " High-quality genomic DNA"). It performs well in amplification and Southern blotting experiments, and is also suitable for archiving (see figure " Efficient downstream PCR analysis").
DNA is purified from 0.1–20 ml (blood), 0.1–2 ml (buffy coat), or 1–2 x 106 cells (cultured cells) in a single tube, processed by centrifugation. Twelve samples can be processed in 1 hour. Depending on sample size, the rehydration volume is 0.2–1 ml.
| Sample | Volume | DNA yield (µg) |
|---|---|---|
| Blood* | 0.3 ml | 11–14 |
| Blood* | 2.0 ml | 75–90 |
| Blood* | 10.0 ml | 380–460 |
| Buffy coat | 0.5 ml | 110–130 |
| HeLa cells | 106 cells | 25–35 |
See figures
Principle
The FlexiGene DNA system enables isolation of genomic DNA from variable amounts of whole blood, buffy coat, and cultured cells. The simple purification procedure is performed in a single tube, which reduces costs and simplifies handling. The purified DNA performs well in sensitive downstream applications.
Procedure
The first step is addition of lysis buffer to the samples (see flowchart " FlexiGene DNA procedure"). Cell nuclei and mitochondria are pelleted by centrifugation and resuspended in denaturation buffer containing QIAGEN Protease. Following protein digestion, DNA is precipitated by addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, and dried. DNA is resuspended in hydration buffer and is ready for direct use in downstream assays or storage at –20ºC. High DNA yields are obtained from whole blood, buffy coat, and cultured cells (see table "DNA yields purified from different sample types using the FlexiGene procedure").
See figures
Applications
DNA purified using the FlexiGene DNA kit performs well downstream applications, including,
- PCR
- Southern blotting
- Archiving
Supporting data and figures
FlexiGene DNA procedure.
The first step is addition of lysis buffer to the samples. Cell nuclei and mitochondria are pelleted by centrifugation and resuspended in denaturation buffer containing QIAGEN Protease. Following protein digestion, DNA is precipitated by addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, and dried. DNA is resuspended in hydration buffer and is ready for direct use in downstream assays or storage at –20ºC.
Specifications
| Features | Specifications |
|---|---|
| applications | PCR techniques, restriction digest, Southern blotting, sequencing |
| sampleamount | 100 µl - 20 ml |
| format | Scalable |
| timeperrunorperprep | 25 - 45 minutes (+ DNA rehydration) |
| mainsampletype | Whole blood, buffy coat, cultured cells |
| technology | Single tube precipitation method |
| processing | Manual |
| yield | 35 µg / ml whole blood |
| purificationoftotalrnamirnapolyamrnadnaorprotein | Genomic DNA |
| elutionvolume | 200 µl - 1000 µl |
Resources
Kit Handbooks (2)
Additional Resources (1)
Supplementary Protocols (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
Publications
The (TAAAA)n microsatellite polymorphism in the SHBG gene influences serum SHBG levels in women with polycystic ovary syndrome.
Hum Reprod; 2006; 22 (4):1031-6 2006 Dec 22 PMID:17189294
A functional SNP of interferon-gamma gene is important for interferon-alpha-induced and spontaneous recovery from hepatitis C virus infection.
Proc Natl Acad Sci U S A; 2007; 104 (3):985-90 2007 Jan 10 PMID:17215375
Lipopolysaccharide-activated CD4+CD25+ T regulatory cells inhibit neutrophil function and promote their apoptosis and death.
J Immunol; 2006; 177 (10):7155-63 2006 Nov 15 PMID:17082633
Predictors of vitamin B6 and folate concentrations in older persons: the InCHIANTI study.
Clin Chem; 2006; 52 (7):1318-24 2006 May 11 PMID:16690736
Past adult lead exposure is linked to neurodegeneration measured by brain MRI.
Neurology; 2006; 66 (10):1476-84 2006 May 23 PMID:16717205