QuickLyse Miniprep Kit for Plasmid DNA Extraction
For fast purification of up to 15 µg sequencing-grade plasmid
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QuickLyse Miniprep Kit (250)
Cat no. / ID. 27406
250 QuickLyse Spin Columns, Reagents, Buffers, Lysis and Collection Tubes (2 ml)
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The QuickLyse Miniprep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Rapid one-step lysis procedure
- Reduced protocol steps and hands-on time
- Up to 15 µg DNA from 1.5 culture volumes obtained
- Ready-to-use plasmid DNA in just 9 minutes
Product Details
The QuickLyse Miniprep Kit provides a rapid spin-column-based method for isolating sequencing-grade plasmid DNA. Bacterial cell resuspension, lysis, and DNA binding are achieved in a single buffer, enabling fast DNA purification with minimal hands-on time.
Performance
The QIAGEN QuickLyse Miniprep Kit uses one-step lysis technology for ultrafast preparation of up to 15 µg plasmid DNA. The quality of the DNA ensures robust performance in applications, such as sequencing, restriction analyses, and cloning (see figure " Reproducible yields").
The QuickLyse Miniprep Kit yields sufficient plasmid DNA for any downstream application, including sequencing (see figure " Long read-lengths in automated sequencing"). Up to 15 µg of high-copy plasmid DNA can be obtained from 1.5 ml of bacterial culture (see figure " High yields of high-quality plasmid DNA"). Isolated plasmid DNA has OD260/280 ratios of 1.7–1.9, indicating that the quality and purity is suitable for all sensitive applications. Sequencing read lengths of over 700 bases are easily achieved.
See figures
Principle
Unlike most protocols that use a 3-step lysis procedure, the QuickLyse Miniprep Kit combines enzymatic and osmotic processes to lyse bacterial cells in a single, 3-minute step. In addition, QuickLyse technology uses fewer buffers, simplifying handling and saving time: 24 plasmid DNA minipreps can be prepared in less than 22 minutes.
Procedure
Bacterial cells are resuspended and lysed and DNA is bound in a single buffer, enabling fast DNA purification with minimal hands-on time. After a 3-minute lysis step, the clear lysate is applied directly to a QuickLyse Spin Column. Plasmid DNA binds to the column membrane and is eluted after a single wash step (see flowchart " QuickLyse procedure"). The isolated DNA can be used directly in standard applications, including automated sequencing, PCR, restriction analysis, and cloning.
See figures
Applications
The QuickLyse Miniprep Kit yields sufficient plasmid DNA for any downstream application, including automated sequencing.
Supporting data and figures
High yields of high-quality plasmid DNA.
Typical DNA yield and purity from 1.5 ml of bacterial culture (LB media) for different cells and vectors.
Specifications
| Features | Specifications |
|---|---|
| applications | Automated sequencing, PCR, restriction analysis, cloning etc. |
| timeperrunorprepperrun | 9 min |
| culturevolumestartingmaterial | 1.5 ml culture volume |
| plasmidtype | High-copy, cosmid DNA |
| elutionvolume | 50 µl |
| samplesperrunthroughput | 1–24 samples per run |
| technology | QuickLyse technology |
| yield | up to 15 µg |
| processing | Manual (centrifugation) |
Resources
Kit Handbooks (1)
Safety Data Sheets (1)
Certificates of Analysis (1)