PAXgene Bone Marrow RNA Kit
For collection of bone marrow samples and stabilization/purification of intracellular RNA
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PAXgene Bone Marrow RNA Kit (30)
Cat no. / ID. 764133
For 30 RNA preps: 30 PAXgene Spin Columns, 30 PAXgene Shredder Spin Columns, Processing Tubes, RNase-free DNase I, RNase-free reagents and buffers. To be used in conjunction with PAXgene Bone Marrow RNA Tubes
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- To be used in conjunction with the PAXgene Bone Marrow RNA Tube
- Rapid purification of RNA with high integrity and purity
- Optimized protocol for heterogeneous bone marrow samples
- Integrated DNase treatment for gDNA removal
Product Details
The PAXgene Bone Marrow RNA System consists of PAXgene Bone Marrow RNA Tubes, for stabilization, storage, and transportation of bone marrow, and the PAXgene Bone Marrow RNA Kit, for silica-membrane-based RNA isolation and purification in a spin-column format. Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is immediately transferred into a PAXgene Bone Marrow RNA Tube, which stabilizes the RNA in the sample for storage at room temperature, refrigerated, or frozen. RNA can be purified days later using the PAXgene Bone Marrow RNA Kit or archived for several months at –20°C or –70°C.
Performance
The PAXgene Bone Marrow RNA Kit enables efficient isolation and purification of intracellular RNA from heterogeneous human whole bone marrow samples (see figure " RNA yields from bone marrow samples"), including an optimized procedure for cell-rich, viscous samples. RNA can be purified days after collection, and intact, high-quality RNA can be purified using the PAXgene Bone Marrow RNA Kit after samples are stored at room temperature, refrigerated, or frozen (see figures " RNA stability at room temperature", " RNA stabilized at 2–8°C", and " High RNA integrity after freeze–thaw cycles"). RNA Tubes protect RNA molecules from degradation by RNases and enable accurate analysis by minimizing ex vivo changes in gene expression (see figure " Stability of 5 transcripts at room temperature"). An integrated DNase treatment ensures that genomic DNA is minimized. In-house and external studies showed that genomic DNA was <0.85% in 95% of the samples tested (n = 157). A260/A280 values are typically between 1.8 and 2.2, indicating the high purity of the RNA (see figure " Storage under different conditions").
See figures
Principle
Copy numbers of individual mRNA species in bone marrow can change significantly during storage or transport at room temperature, making reliable studies of gene expression impossible. PAXgene Bone Marrow RNA Tubes protect RNA molecules from degradation and immediately stabilize the RNA in the sample for storage at room temperature, refrigerated, or frozen.
Procedure
Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is then transferred into a PAXgene Bone Marrow RNA Tube. The RNA in the sample is immediately stabilized for storage at room temperature, refrigerated, or frozen. The PAXgene Bone Marrow RNA Kit provides an easy and rapid procedure for isolation and purification of intracellular RNA from human whole bone marrow samples. The kit uses silica-membrane technology in a spin-column format for efficient purification of intact, high-quality RNA.
Applications
RNA purified using the PAXgene Bone Marrow RNA Kit is ready for use in a wide range of downstream applications, including
- Real-time RT-PCR analysis of specific transcripts
- Array analysis of complex expression profiles
Supporting data and figures
Stability of 5 transcripts at room temperature.
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Specifications
| Features | Specifications |
|---|---|
| applications | RT-PCR, array analysis |
| yield | 11 µg |
| timeperrun | 95 min/12 samples |
| mainsampletype | Bone marrow samples |
| sampleamount | 2 ml |
| processing | Manual (centrifugation) |
| format | Spin column |
| elutionvolume | 80 µl (2 x 40 µl) |
| technology | Silica technology |
Resources
Brochures & Guides en (2)
Safety Data Sheets (1)
Scientific Posters (2)
Gene Expression Analysis (1)
Kit Handbooks (2)
Certificates of Analysis (1)