QIAcuity Probe PCR Kit

QIAcuityデジタルPCR専用試薬

✓ オンライン注文による24時間年中無休の自動処理システム

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✓ 迅速で信頼性の高い（再）注文

QIAcuity Probe PCR Kit (5 ml)

カタログ番号 / ID. 250102

4x QIAcuity プローブマスターミックス(1mL、5本)、滅菌水（1.9 ml、8本）

￥121,000

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容量

5 ml

1 ml

25 ml

数量

QIAcuity Probe PCR Kitは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ オンライン注文による24時間年中無休の自動処理システム

✓ 知識豊富で専門的な製品&テクニカルサポート

✓ 迅速で信頼性の高い（再）注文

特徴

最大5色蛍光を利用したマルチプレックスPCR に対応
より多くのサンプルを利用できる4x 濃度のマスターミックス
QIAcuity ナノプレートに最適化
非特異的PCR 増幅を低減
REACH 準拠

製品詳細

QIAcuityプローブPCRキットには、QIAcuityナノプレートに最適化された、4x マスターミックスが含まれています。このキットは、プローブベースのデジタルPCRの特異性と効率を高め、正確なシングルプレックス解析から最大5プレックス解析までをカバーします。 QIAcuityプローブマスターミックスは、QIAcuity dPCR装置でのgDNAまたはcDNAの正確な定量のための特異性を高めるように最適化されています。

本キットは QIAcuity デジタルPCR システムおよび QIAcuity ナノプレートと組み合わせて使用します。

製品について詳しくお知りになりたい場合は、弊社のdPCRスペシャリストから連絡を差し上げますので、こちらからサインインしてください。

パフォーマンス

優れたパフォーマンス
加水分解プローブを使用した本QIAcuityマスターミックスは、QIAGENの最新のDNAポリメラーゼを採用しています。新しいQuantiNova DNA Polymeraseとナノプレートに最適化されたQIAGEN独自のバッファーテクノロジーの組み合わせは、感度、再現性、効率において、誤差の少ない結果を提供します。

加水分解プローブによるマルチプレックスPCR
QIAcuity プローブPCRキットの特別なマスターミックスは、QIAcuityナノプレート上のウェルにおいて、最大5倍のターゲットの量の差を正確に定量することができます。これにより、時間とコストを節約し、必要なサンプルの量を減らすこともできます。さらに、得られた二重または多重PCRデータは、シングルプレックスPCRから得られたものと同等です。

最大100時間までの反応安定性
QIAcutiy PCRミックスは、その後の反応のパフォーマンスを損なうことなく、30℃で最大100時間保存できます。この優れた安定性は、冷却剤を使用せずに室温で長期間保管できるため、ハイスループット処理でのサンプルセットアップやプレートスタッカーでの自動化にも対応できます。

原理

QIAcuity プローブPCRキットは、新しい抗体を使ったホットスタート機構により、シングルプレックスまたはデュプレックスでのcDNAまたはgDNA解析を高い特異性をもって実現します。低温でQuantiNova DNAポリメラーゼは、QuantiNova抗体および新しい添加剤QuantiNova Guardによって不活化状態を維持します。これにより、ホットスタートでの不活化状態がより向上し、非特異的にアニーリングしたプライマーおよびプライマーダイマーの伸長を強く抑制します。温度を95°Cに上げると、2分以内にQuantiNova抗体とQuantiNova Guardは変性し、QuantiNova DNAポリメラーゼが活性化され、PCR増幅が可能になります。

ナノプレートでのｄPCRの詳細は、こちら

操作手順

qPCR実験のようにサンプル調整には、マスターミックス、プローブ、プライマーを96または24ウェルナノプレートへ加え、その後サンプルを加えるステップは存在しますが、このシステムでは、サンプルの分配、PCR増幅およびイメージングを1台の装置の中で自動化し、サンプルから結果までを2時間以内に完了します。ソフトウェアは、ターゲットシーケンスのマイクロリットルあたりのコピー数を計算するだけでなく、ポジティブサンプルやNTCなどのコントロールも同時に解析します。またこの解析は、同じローカルエリアネットワーク（LAN）内の離れた場所のPCでも行えます。

アプリケーション

QIAcuity Probe PCR Kitは、QIAcuityデジタルPCRシステムおよびQIAcuity ナノプレートとともに、以下のアプリケーションでのcDNAやgDNAの定量的解析に利用できます。

希少な突然変異の検出
コピー数多型解析
遺伝子発現解析
病原菌の検出
ジェノタイピング
miRNA研究

裏付けデータと数値

プレート方式のシンプルで迅速なワークフロー

パーティショニング、サーマルサイクル、イメージングを1つに統合した完全自動化装置が2時間未満で結果を提供します。ナノプレート方式はフロントエンドのサンプル調製の自動化に適しており、手作業時間をさらに短縮できます。

リソース

For Version 2.0

For Version 2

Version 4.0

The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.

After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.

Required QIAcuity Software Suite version: Version 1.2 or higher.

Version 2.1

Version 1.2

The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

The following browsers are supported in the QIAcuity Software Suite:

-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)

The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.

Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.

The new improvements are as follows:

-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting

For Version 1.2

For Version 1.0

Fast. Scalable. Reliable.

For use with QIAcuity systems

July 2021

This study tested a workflow for quantitation and qualification of AAV samples using a duplex assay on the QIAcuity dPCR instrument targeting both an insert (GFP) and the viral backbone (AAV2-ITR). With very low intra-assay and inter-assay CVs <6.5%, we demonstrate one of the main benefits of dPCR: reproducibility.

The goal of this work was to compare performance of quantitative PCR (qPCR) and digital PCR (dPCR) in the quantification of gene expression and Wolbachia abundances in Nasonia parasitoid wasps.

Here we provide an integrated rAAV genome titer method using the QIAcuity Digital PCR (dPCR) System with detailed parameters for high assay performance. Using this optimized method for pre-PCR handling of in-process rAAV samples, the results demonstrated that QIAcuity dPCR system generates the same level of accuracy and precision as the current gold standard ddPCR system but with much faster sample-to-result times (2 hours vs 7 hours) and higher overall throughput and scalability.

Limitations of conventional PCR and qPCR when dealing with difficult, low-volume samples and complex mixtures with high background of competitive molecules and inhibitors have posed frequent challenges for researchers and clinicians in their routine work. With the new generation of PCR technologies, digital PCR has opened doors for diverse applications, and researchers are learning to ask questions only digital PCR can answer. Join QIAGEN's webinar on how digital PCR can help take your research applications through and beyond those challenges.

This presentation will introduce dPCR, discuss its advantages, and outline how the approach might be used to improve measurement in areas like clinical diagnosis, alone or in conjunction with other methods.

In this expert webinar, Dr. Kubista will share with you the experience he and his team have gathered at the TATAA Biocenter, developing applications and providing services using digital PCR for nearly 12 years. They have experienced all the problems common to dPCR analytical workflows and developed robust standard operating procedures to minimize the risk of error and maximize robustness and repeatability, and developed various controls to test the performance and validate the methods. He will also discuss dPCR assay design and validation and then focus on strategies for copy number determination and rare mutation detection.

The QIAGEN digital PCR technology and its expanded capability will not only transform the portfolio of conventional qPCR applications but also provide a more rapid, accurate, and sensitive method for finding answers to difficult biological questions.

As the digital PCR technology evolves and becomes more accessible and affordable, the transition from qPCR and adoption of dPCR will hopefully no longer remain a challenge. Experts share insights in an upcoming webinar about the fully integrated, rapid, and highly flexible digital PCR portfolio from QIAGEN.

Fast. Scalable. Reliable.

Version 1.0

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.

This new version of the control software includes system stability and performance reliability improvements. We highly recommend to update the control software of your QIAcuity instrument.

Note: The installation requires approximately 90 minutes.

Version 2.0

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis

Besides system stability and performance reliability improvements, this new version of the control software offers advanced user management and audit trail functionalities. Together with the QIAcuity Software Suite 2.0, the QIAcuity systems now support 21 CFR Part 11 regulations for users working under GMP.

Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.

Note: Upgrading the QIAcuity Control Software to version 2.0 will require upgrading the QIAcuity Software Suite to version 2.0.

Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.0. It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!

Version 2.1

Beside of improvements and bug fixes, the new features mainly address the GMP requirements by offering additional support for 21 CFR part 11 compliance and get the software ready for future Nanoplate support.

For more information, please refer to the Release Note: QIAcuity Instrument Control Software, Version 2.1.

SHA1 checksum: 0DD41A8DE46D10E81F538ACB71AC184170D39FE0

Version 1.1.3

Version 1.2

-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)

Version 2.0

The QIAcuity Software Suite 2.0 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

This new software version now supports 21 CFR Part 11 regulations for users working under GMP. This includes the following:

-Advanced User Management
-Audit Trail
-Signatures

Additional changes compared with the previous software version include the following:

-Usability improvements
-Improved dust detection
-Hyperwell expansion to CNV and GEX analysis
-CSV export functionality of multiple positive partitions for linkage analysis, drop-off assays, etc.
-Raw data export

Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.

Important: A direct update of the Software Suite from version 1.1.3 to 2.0.20 is not possible. Please follow the instructions in the User Manual on how to update to version 1.2.18 first before updating the Software Suite to version 2.0.20.

Before you start upgrading to version 1.1.3, please make sure that all plates are imported and visible in the plate overview of the QIAcuity Software Suite.

Caution: Not following the instructions may result in a loss of your previous plate data!

Please contact QIAGEN Technical Services if you are unsure and require technical support.

SHA1 checksum: C60C4EEC349DF1C037796016D32A93EEC19B54A0

Version 2.1

Beside of usability improvements and bug fixes, the new features address GMP requirements by offering additional support for 21 CFR part 11 compliance, copy number variation (CNV) analysis, support of future Nanoplates, and improved 1D scatterplot views.

For more information, please refer to the Release Note: QIAcuity Software Suite, Version 2.1

SHA1 checksum: 6D51E273B26FA7D7E0EDA843CB3DCD74B38FA5B1

For Version 1.0

For Version 1.2

For Version 2.0

For Version 2

Version 2.1

For use with QIAcuity systems

July 2021

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QIAcuity Probe PCR Kit

QIAcuityデジタルPCR専用試薬

QIAcuity Probe PCR Kit (5 ml)

特徴

製品詳細

パフォーマンス

原理

操作手順

アプリケーション

裏付けデータと数値

プレート方式のシンプルで迅速なワークフロー

リソース