Products
RT2 SYBR® Green qPCR Mastermixes are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
RT² SYBR Green Fluor qPCR Mastermix (24)
Cat. No. / ID. / ID. 330513
Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x10 µl reactions
RT² SYBR Green qPCR Mastermix (12)
Cat. No. / ID. / ID. 330502
Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions
RT² SYBR Green ROX qPCR Mastermix (24)
Cat. No. / ID. / ID. 330523
Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x 10 µl reactions
RT² SYBR Green qPCR Mastermix (2)
Cat. No. / ID. / ID. 330500
Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Arrays or 200 x 25 µl reactions or 400 x 10 µl reactions
RT² SYBR Green ROX qPCR Mastermix (25 ml)
Cat. No. / ID. / ID. 330529
Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactions or 5000 x 10 µl reactions
RT² SYBR Green Fluor qPCR Mastermix (12)
Cat. No. / ID. / ID. 330512
Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions
RT² SYBR Green qPCR Mastermix (24)
Cat. No. / ID. / ID. 330503
Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x 10 µl reactions
RT² SYBR Green ROX qPCR Mastermix (6)
Cat. No. / ID. / ID. 330524
Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Array or 600 x 25 µl reactions or 1500 x 10 µl reactions
RT² SYBR Green Fluor qPCR Mastermix (2)
Cat. No. / ID. / ID. 330510
Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactions or 400 x 10 µl reactions
RT² SYBR Green qPCR Mastermix (25 ml)
Cat. No. / ID. / ID. 330509
Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactions or 5000 x 10 µl reactions
RT² SYBR Green ROX qPCR Mastermix (8)
Cat. No. / ID. / ID. 330521
Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions
RT² SYBR Green qPCR Mastermix (8)
Cat. No. / ID. / ID. 330501
Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions
RT² SYBR Green Fluor qPCR Mastermix (25 ml)
Cat. No. / ID. / ID. 330519
Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactions or 5000 x 10 µl reactions
RT² SYBR Green Fluor qPCR Mastermix (6)
Cat. No. / ID. / ID. 330514
Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Array or 600 x 25 µl reactions or 1500 x 10 µl reactions
RT² SYBR Green ROX qPCR Mastermix (12)
Cat. No. / ID. / ID. 330522
Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions
RT² SYBR Green Fluor qPCR Mastermix (8)
Cat. No. / ID. / ID. 330511
Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions
RT² SYBR Green qPCR Mastermix (6)
Cat. No. / ID. / ID. 330504
Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Arrays or 600 x 25 µl reactions or 1500 x 10 µl reactions
RT² SYBR Green ROX qPCR Mastermix (2)
Cat. No. / ID. / ID. 330520
Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactions or 400 x 10 µl reactions
Features
- Designed exclusively for use with RT2 and EpiTect Methyl II qPCR Assays
- Delivers specific amplification with minimal primer-dimers
- Available with ROX, fluorescein, or without dye
- For use with qPCR instruments under standard cycling conditions
Product Details
RT2 SYBR Green qPCR Mastermixes are highly suited for real-time PCR applications using SYBR Green, and are required for guaranteed qPCR performance of the RT2 qPCR Primer Assays, RT2 Profiler PCR Arrays, RT2 lncRNA qPCR Assays, and RT2 lncRNA PCR Arrays. This Mastermix should be used in qPCR instruments under standard cycling conditions. RT2 SYBR Green qPCR Mastermixes are also suitable for use with EpiTect ChIP and EpiTect Methyl II qPCR Assays and PCR Arrays.
Performance
RT² SYBR Green qPCR Mastermixes contain all of the optimized reagents and buffers needed for SYBR Green based real-time PCR with RT2 qPCR Primer Assays, RT2 Profiler PCR Arrays, RT2 lncRNA qPCR Assays, and RT2 lncRNA PCR Arrays on all real-time PCR instruments.
The high-performance real-time PCR enabled by RT² SYBR Green qPCR Mastermixes demonstrates high amplification efficiencies (see figure " High amplification efficiency over a wide dynamic range") and high levels of sensitivity and specificity (see figure " Tighter control of polymerase activity yields greater specificity").
See figures
Principle
RT2 SYBR Green qPCR Mastermixes contain real-time PCR buffer, a high-performance, HotStart DNA Taq polymerase, nucleotides, and SYBR Green dye. Some mastermixes contain either fluorescein or ROX dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate SYBR Green results by preventing the amplification of primer–dimers and other nonspecific products.
Mastermixes without ROX or fluorescein
RT2 SYBR Green qPCR Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation not requiring a reference dye, including Bio-Rad models CFX96, CFX384; Bio-Rad/MJ Research Chromo4, DNA Engine Opticon, DNA Engine Opticon 2; Roche LightCycler 480 (96-well and 384-well); Eppendorf Mastercycler ep realplex without ROX filter set; and Cepheid SmartCycler.
Mastermixes with fluorescein
RT2 SYBR Green Fluor qPCR Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation that uses fluorescein as a reference dye, including the Bio-Rad models iCycler, iQ5, MyiQ, and MyiQ2.
Mastermixes with ROX
RT2 SYBR Green ROX qPCR Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation that uses ROX as a reference dye, including QIAGEN’s Rotor-Gene Q; Applied Biosystems models 5700, 7000, 7300, 7500 (Standard and Fast), 7700, 7900HT (Standard and Fast 96-well block, 384-well block), StepOnePlus, ViiA 7 (Standard and Fast 96-well block, 384-well block); Eppendorf Mastercycler ep realplex with or without ROX filter set; Stratagene models Mx3000P, Mx3005P, Mx4000; and Takara TP-800.
Applications
RT2 SYBR Green qPCR Mastermixes are suitable for use in real-time PCR applications, especially RT2 qPCR Primer Assays, RT2 Profiler PCR Arrays, RT2 lncRNA qPCR Assays, and RT2 lncRNA PCR Arrays.
Supporting data and figures
High amplification efficiency over a wide dynamic range.
High amplification efficiency over a wide dynamic range.
Resources
Safety Data Sheets (1)
Application Notes (1)
Instrument Technical Documents (1)
Kit Handbooks (1)
Certificates of Analysis (1)