Products
The QIAamp Virus BioRobot MDx Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
QIAamp Virus BioRobot MDx Kit (12)
Cat. No. / ID. / ID. 965652
For 12 x 96 preps for use on the BioRobot MDx; includes: 12 QIAamp 96 Plates, RNase-free buffers, QIAGEN Protease, Elution Microtubes CL, caps, S-Blocks, carrier RNA
Features
- Rapid isolation of high-quality, ready-to-use viral DNA and RNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Removal of contaminants and inhibitors
Product Details
The QIAamp Virus BioRobot MDx Kit provides automated viral nucleic acid purification on the BioRobot MDx using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.
Performance
The QIAamp Virus BioRobot MDx process provides uniform DNA and RNA recovery and purity across the 96-well plate with no detectable cross-contamination between samples. Up to 265 µl of cell-free body fluids can be processed. The typical elution volume is 75 µl. Viruses can be detected with high sensitivity when using this kit, which is illustrated by hit-rate studies (see table). Further advantages of the system include high process reliability and walkaway automation, providing a highly efficient system for purification of viral nucleic acids. The QIAamp Virus BioRobot MDx provides reliable results in downstream applications.
| PCR product (n=96) | Instrument 1 | Instrument 2 | ||||
| Mean CT | Standard deviation | %CV | Mean CT | Standard deviation | %CV | |
|---|---|---|---|---|---|---|
| Mimic virus | 28.26 | 0.10 | 0.35 | 28.38 | 0.18 | 0.65 |
| Internal control | 31.98 | 0.16 | 0.51 | 31.87 | 0.22 | 0.70 |
Principle
The QIAamp Virus BioRobot MDx Kit simplifies isolation of viral RNA and DNA from cell-free body fluids with a fast automated 96-well–plate procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp technology yields viral RNA and DNA from cell-free body fluids ready to use in PCR and blotting procedures.
The fully automated procedure, including bar code reading and complete process documentation, requires less than 2.5 hours to process 96 samples (including a detailed load check of 20 minutes) with no hands-on time during the run. Advantages of the system include high process reliability and walkaway automation, providing a highly efficient system for purification of viral nucleic acids for laboratories. QIAamp sample preparation technology is fully licensed.
Procedure
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use nucleic acids are then eluted in water or low-salt buffer (see flowchart " Protocol").
See figures
Applications
The QIAamp Virus BioRobot MDx Kit provides automated, high-throughput purification of viral RNA and DNA on the BioRobot MDx using proven QIAamp technology. The 96-well plates provide the flexibility of processing 32–96 samples, in increments of 8. Sample sources include:
- Plasma and serum
- CSF
- Other cell-free body fluids
- Cell-culture supernatants
Supporting data and figures
QIAamp Virus BioRobot MDx protocol.
QIAamp Virus BioRobot MDx protocol.
Specifications
| Features | Specifications |
|---|---|
| applications | PCR, qPCR, real-time PCR |
| samplesperrunthroughput | 36-92 samples per run |
| technology | Silica technology |
| mainsampletype | Serum, plasma |
| elutionvolume | 75 µl |
| processing | Automated |
| forautomatedprocessing | BioRobot MDx Workstation |
| sampleamount | 265 µl |
| format | 96-well plates |
| purificationoftotalrnamirnapolyamrnadnaorprotein | Viral DNA, viral RNA |
| timeperrunorperprep | <2.5 hours |
| yield | >90% recovery |
Resources
Brochures & Guides en (1)
Safety Data Sheets (1)
Kit Handbooks (1)
Brochures & Guides (1)
Certificates of Analysis (1)