QIAamp Investigator BioRobot Kit
For automated medium- to high-throughput purification of forensic DNA on the BioRobot Universal System
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QIAamp Investigator BioRobot Kit (12)
Cat no. / ID. 965942
For 12 x 96 DNA preps: QIAamp 96 Plates, S-Blocks, Buffers, Proteinase K, Elution Microtubes CL, Caps, Disposable Troughs
The QIAamp Investigator BioRobot Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- DNA purification from forensic and human identity samples
- More efficient yields from trace casework samples
- Higher signal-to-noise ratios
- Rapid isolation of high-quality, ready-to-use DNA
Product Details
The QIAamp Investigator BioRobot Kit provides automated DNA purification from up to 96 samples (such as filters, casework or crime-scene samples, and blood) or up to 192 swabs on the BioRobot Universal System using proven QIAamp silica-membrane technology. The fully automated procedure on the BioRobot Universal System requires less than 3 hours, including bar code reading, sample tracking and complete process documentation, with no hands-on time during the run. The purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios.
Performance
The QIAamp Investigator BioRobot Kit is suited for many applications, such as:
- Purification of DNA from forensic casework and database samples
- DNA purification from different trace samples (e.g., crime-scene)
- Routine analysis of reference samples
Principle
The BioRobot Universal System together with the Application Pack, Investigator and the QIAamp Investigator Kit meet the demands of forensic laboratories with flexible, fully automated, standardized processing using proven QIAamp chemistry.
Procedure
The robust purification procedure provides high process safety and helps to prevent cross-contamination. For increased convenience and flexibility, there is the option of walkaway sample lysis and DNA purification or, if required, lysis can be performed manually followed by walkaway purification of DNA. The fully automated procedure on the BioRobot Universal System requires less than 3 hours, including bar code reading, sample tracking and complete process documentation, with no hands-on time during the run.
Applications
The purification procedure of the QIAamp Investigator BioRobot Kit provides high-quality DNA from 24 to 96 forensic samples in batches of 8. Using the kit, the following samples can be processed together in a single run:
- Dried blood
- Surface swabs (e.g., blood, semen, saliva, and epithelial cells)
- Stains on fabric (e.g., blood- or saliva-stained fabrics)
- Cigarette butts and tobacco
- Chewing gum and envelopes
Supporting data and figures
High-quality DNA from a wide range of sample types.
DNA was purified from the indicated sample types using the QIAamp Investigator BioRobot Kit (Investigator) on the BioRobot Universal System, or manually using the QIAamp DNA Micro Kit (Micro). Aliquots (5 μl, 10%) of each eluate were analyzed in triplicate by quantitative real-time PCR using the QuantiTect Probe PCR Kit with primers and probe specific for the β-actin gene. The mean threshold value (CT) and standard deviation are indicated for each sample.
Specifications
| Features | Specifications |
|---|---|
| applications | STR Typing, PCR, blotting |
| timeperrunorperprep | <3 hours |
| yield | 1-2 µg DNA per swab; varying for casework samples |
| forautomatedprocessing | Biorobot Universal platform |
| processing | Automated |
| sampleamount | 1 swab per well, up to 30 mg chewing gum, up to 40 mg cigarette butts, envelops, fabric or hair |
| mainsampletype | Forensic casework and human identity samples |
| elutionvolume | 150 µl |
| purificationoftotalrnamirnapolyamrnadnaorprotein | Genomic DNA |
| format | 96-well plate |
| technology | Silica technology |
| samplesperrunthroughput | 24-96 samples per run |
Resources
Safety Data Sheets (1)
Kit Handbooks (1)
Certificates of Analysis (1)