RT² qPCR Primer Assays

• Complete genome coverage for multiple species
• Amplifies a single amplicon with uniform PCR efficiency
• Less than 5 minutes hands-on time

RT² qPCR Primer Assays are specifically designed and experimentally verified for real-time PCR analysis. The rigorous assay verification criteria ensure PCR specificity and efficiency for reliable and accurate gene expression analysis results.

RT² qPCR Primer Assays use SYBR^® Green-based quantitative real-time PCR technology to provide a sensitive and reliable tool for gene expression analysis.

Each assay utilizes a proprietary and experimentally verified algorithm for the design of gene-specific qPCR primers with uniform PCR efficiency and amplification conditions. Each lot of every assay is further wet-bench tested for real-time PCR performance for specificity and amplification efficiency. Amplification of a single product of the correct size with high PCR efficiency (>90%) is guaranteed when the assays are used with RT² SYBR^® Green qPCR Mastermixes. The uniform PCR efficiencies and PCR conditions of the RT² qPCR Primer Assays provide an accurate and scalable solution for multiple gene expression analyses.