QIAcuity Nanoplatesおよびアクセサリー

QIAcuityデジタルPCR装置で使用

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✓ 迅速で信頼性の高い（再）注文

QIAcuity Nanoplate 26k 8-well (10)

カタログ番号 / ID. 250031

10 QIAcuity Nanoplate 26k 8-well, 11 Nanoplate Seals

€288.00

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Product TypeAccessories

QIAcuity Nanoplate

Nanoplate Seals

Nanoplate Tray

Nanoplate Adapter

ウェルあたりのパーティション

26k

8.5k

ウェル数

数量

QIAcuity Nanoplatesおよびアクセサリーは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ オンライン注文による24時間年中無休の自動処理システム

✓ 知識豊富で専門的な製品&テクニカルサポート

✓ 迅速で信頼性の高い（再）注文

特徴

さまざまなアプリケーションのニーズに対応した4種類のナノプレート
1ウェルあたり8500分画または26,000分画
SBS規格に準拠

製品詳細

QIAcuity NanoplatesはデジタルPCR用のマイクロ流体プレートです。1ウェルあたりの分画数は8,500または26,000で最大8サンプル、24サンプルまたは96サンプルを処理できます。4種類のナノプレートはすべて、QIAcuity Digital PCR Systemでの使用を想定して設計されています。

これらのナノプレートは、QIAcuity Digital PCR Systemでのみ使用できます。QIAgilityを使用してQIAcuity Nanoplatesにおける液体の取り扱いとPCRセットアップを自動化するには、専用のQIAcuity Nanoplate Adapterをご使用ください。アダプターを取り付け後、QIAcuity Digital PCR SystemにプレートをロードしてdPCRを実施します。

製品について詳しくお知りになりたい場合は、弊社のdPCRスペシャリストより連絡を差し上げますので、こちらからサインインしてください。

パフォーマンス

QIAcuity装置によるデジタルPCR用に特別に設計されたプレートです。アプリケーションの用途に応じた仕様を満たし、SBS規格に準拠した4種類のナノプレートを提供しています。

Nanoplates – 機能と仕様
タイプ	フレームカラー	仕様	アプリケーション
Nanoplate 26K 8-well	ライトブルー	8ウェル、約26,000分画 1ウェルあたり容量　40 µl	希少突然変異検出、リキッドバイオプシー、遺伝子発現解析、病原体検出等
Nanoplate 26K 24-well	ブルー	24ウェル、約26,000分画 1ウェルあたり容量　40 µl	希少突然変異検出、リキッドバイオプシー、遺伝子発現解析、病原体検出等
Nanoplate 8.5K 24-well	ホワイト	24ウェル、約8,500分画 1ウェルあたり容量　12 µl	コピー数多型解析、遺伝子発現解析、 NGSライブラリー定量、ゲノム編集検出等
Nanoplate 8.5K 96-well	グレー	96ウェル、約8,500分画 1ウェルあたり容量　12 µl	コピー数多型解析、遺伝子発現解析、 NGSライブラリー定量、ゲノム編集検出等

原理

分注およびロード、実験、結果解析というわずか3つのステップで、2時間以内にdPCRの結果が得られます。ナノプレートでのdPCRの原理はこちらで説明しています。

操作手順

qPCR実験と同様に、サンプル調製では、マスターミックス、プローブ、プライマーを8、24または96ウェルナノプレートに加え、その後サンプルを加えます。このシステムは、パーティショニング、サーマルサイクル、イメージングを1つに統合した完全自動化装置です。サンプルから結果まで2時間未満で完了します。Software Suiteは、ターゲット配列のマイクロリットルあたりのコピー数で濃度を提供するだけでなく、陽性サンプルやNTCなどの品質コントロールも解析できます。この解析は、同じローカルエリアネットワーク（Local Area Network、LAN）内の離れた場所にあるコンピューターでも行うことができます。

アプリケーション

QIAcuity Nanoplatesは、QIAcuityデジタルPCRシステムおよびQIAcuity PCRキットと組み合わせて、次のデジタルPCRアプリケーションに利用できます。

希少突然変異の検出
コピー数多型解析
遺伝子発現解析
病原体検出
遺伝子型決定
miRNA研究
遺伝子細胞治療
残留DNA定量
排水モニタリング

リソース

For Version 2.0

For Version 2

Version 4.0

The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.

After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.

Required QIAcuity Software Suite version: Version 1.2 or higher.

Version 2.1

Version 1.2

The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

The following browsers are supported in the QIAcuity Software Suite:

-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)

The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.

Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.

The new improvements are as follows:

-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting

For Version 1.2

For Version 1.0

The QIAgility instrument is a liquid handler designed for automating PCR setup. For compatibility with the QIAcuity, we developed an adapter to secure up to two nanoplates onto the deck of the QIAgility. Using the QIAgility software, we have optimized a protocol that works for all nanoplate types and QIAcuity applications. Here we report the performance of a front end automated QIAgility dPCR nanoplate setup procedure for use with the QIAcuity dPCR system.

The QIAcuity digital PCR system combined with the QIAcuity One-Step Viral RT-PCR Kit enables precise detection and quantitation of vector-borne viruses in mosquitoes. The results presented in this comparison study showed that digital PCR is a powerful tool for absolute quantitation of low abundant targets and is a more reliable detection method than qPCR. Multiplexing allows detection and quantitation of multiple targets in a single reaction more efficiently by increasing sample throughput at a reduced cost per target.

Digital PCR is a superior method to qPCR for the detection and absolute quantification of low concentration target templates. There are multiple digital PCR systems on the market that differ in numerous aspects including the amount of dead volume, which is the volume that is loaded but not analyzed by the given instrument. While it has been speculated that dead volume could impact the sensitivity of dPCR applications, here we provide data to support the conclusion that the most important factors in determining the relative sensitivity of each system are template addition volume and template analyzed volume. In summary, data provided herein demonstrate that higher template addition volumes can overcome any limitations that dead volume may have on the sensitivity of a dPCR application.

The goal of this work was to compare performance of quantitative PCR (qPCR) and digital PCR (dPCR) in the quantification of gene expression and Wolbachia abundances in Nasonia parasitoid wasps.

This study tested a workflow for quantitation and qualification of AAV samples using a duplex assay on the QIAcuity dPCR instrument targeting both an insert (GFP) and the viral backbone (AAV2-ITR). With very low intra-assay and inter-assay CVs <6.5%, we demonstrate one of the main benefits of dPCR: reproducibility.

Here we report the use of saliva samples in combination with dPCR as a suitable alternative to screen for individuals infected with SARS-CoV-2.

Here we demonstrate how to optimize your assays on a microfluidic nanoplate-based digital PCR system, the QIAcuity, and provide recommendations for a seamless transfer. Moreover, the QIAcuity dPCR workflow is very similar to qPCR.

Here we compared the performance of qPCR and the nanoplate dPCR techniques. The digital PCR method on the QIAcuity significantly improved precision when measuring copy number states and sensitivity of mutation detection through absolute quantification and reduced standard error. This is advantageous in various applications, including copy number variation analysis, small fold-change and rare mutation detection.

Here we provide an integrated rAAV genome titer method using the QIAcuity Digital PCR (dPCR) System with detailed parameters for high assay performance. Using this optimized method for pre-PCR handling of in-process rAAV samples, the results demonstrated that QIAcuity dPCR system generates the same level of accuracy and precision as the current gold standard ddPCR system but with much faster sample-to-result times (2 hours vs 7 hours) and higher overall throughput and scalability.

Fast. Scalable. Reliable.

For use with QIAcuity systems

July 2021

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