mRNA transfection 또는 plasmid DNA와 siRNA를 동시에 효율적으로 transfection할 수 있습니다
✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
TransMessenger Transfection Reagent (0.5 ml)
카탈로그 번호 / ID. 301525
For 60 transfections in 6-well plates or 80 transfections in 12-well plates
로그인 계정 가격 보기.
TransMessenger Transfection Reagent은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
특징
- 효율적인 RNA transfection
- siRNA-plasmid DNA co-transfection을 이용한 gene silencing 실험에 매우 적합
- 빠르고 편리한 setup
제품 세부 정보
TransMessenger Transfection Reagent is a ready-to-use, lipid-based reagent for RNA transfection of eukaryotic cells. Transfection of cells with RNA rather than DNA offers new possibilities for transfection experiments. Cell lines successfully transfected with TransMessenger Reagent include 293T, Jurkat, and Vero. This product can also be used for RNA transfection of primary human fibroblasts, rat cardiomyocytes, and rat neuronal cells.
성능
TransMessenger Reagent provides a fast and easy procedure and high transfection efficiencies (see figure " TransMessenger Reagent with HeLa cells"). Since the amount of RNA is a critical factor for successful transfection, we recommend optimizing the amounts of RNA and TransMessenger Transfection Reagent for every cell type–RNA combination (see figure " Amount of RNA and TransMessenger Reagent vs. transfection efficiency"). To facilitate this, the reagent is provided with guidelines for optimization together with starting points for optimization in different cell-culture formats.
그림 참조
원리
TransMessenger Transfection Reagent is the first reagent specifically developed for transfection of cells with RNA. The reagent is a lipid-based formulation that is used in conjunction with a specific RNA-condensing enhancer and an optimized buffer. RNA molecules are condensed by the enhancer and then coated by TransMessenger Reagent for efficient transfer into eukaryotic cells. Strict quality control is performed to test for absence of RNase activity, lot-to-lot consistency, and low endotoxin levels (≤10 EU/ml). Our rigorous standards eliminate reagent variables that can adversely affect the efficiency of RNA transfection.
절차
All TransMessenger Reagent components are provided as ready-to-use solutions. To generate TransMessenger–RNA transfection complexes, simply mix your RNA with Enhancer R and Buffer EC-R and incubate for 5 minutes at room temperature, then add TransMessenger Reagent and incubate for a further 5–10 minutes. The complexes are mixed with medium and added directly to the cells. Following a 3 hour incubation, the medium is changed and the cells are incubated until they are ready for analysis.
Optimal transfection results are achieved using high-purity RNA that is free of DNA, proteins, and other contaminants. RNA purified with RNeasy and Oligotex mRNA Kits is highly recommended.
Optimal transfection results are achieved using high-purity RNA that is free of DNA, proteins, and other contaminants. RNA purified with RNeasy and Oligotex mRNA Kits is highly recommended.
응용 분야
Transfection of cells with RNA rather than DNA offers new possibilities for transfection experiments. Transfected RNA sequences are expressed in the absence of transcription, and in a promoter-independent manner. In addition, protein expression usually occurs sooner following transfection of RNA rather than DNA. RNA transfection with TransMessenger Transfection Reagent could be used for:
- Studies of cells not efficiently transfected with plasmid DNA
- Direct studies of RNA function
지원되는 데이터 및 수치
Amount of RNA and TransMessenger Reagent vs. transfection efficiency.
Optimization experiments were performed in CHO-K1 cells. Cells (2 x 104) were seeded in quadruplicate into 96-well plates and transfected 24 hours later with an in vitro-transcribed CAT-encoding RNA using [A] increasing amounts of RNA with 1.5 µl TransMessenger Reagent and [B] increasing amounts of TransMessenger Reagent with 0.25 µg RNA, as described in the TransMessenger Transfection Reagent Handbook. CAT activity was measured 24 hours post-transfection.
사양
| 특징 | 사양 |
|---|---|
| applications | Direct studies of RNA function |
| features | Transfection with RNA. Efficient transfection of neuronal cells. |
| controls | Not included |
| nucleicacid | RNA |
| celltype | Eukaryotic cells |
| numberofpossibletransfections | 80 transfections in 12-well plates / 0,5 ml reagent |
| technology | Lipid-based formulation in conjunction with a RNA-condensing enhancer |
| transfectiontype | Transient transfection, co-transfection |
리소스
Transfection Protocols (2)
Kit Handbooks (1)
Brochures & Guides en (2)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Supplementary Protocols (1)
Brochures & Guides (1)
Certificates of Analysis (1)