QIAamp Virus BioRobot 9604 Kit

BioRobot 9604 workstation을 이용하여 cell-free body fluids에서 viral nucleic acids를 자동으로 분리 정제할 수 있습니다

Products

QIAamp Virus BioRobot 9604 Kit은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
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QIAamp Virus BioRobot 9604 Kit (12)

Cat. No. / ID. / ID.   965662

For 12 x 96 nucleic acid preps: 12 QIAamp 96 Plates, RNase-free buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), carrier RNA, caps
US$7,320.00

특징

  • High-quality의 신속한 분리 정제, ready-to use viral DNA and RNA
  • Organic extraction 또는 alcohol precipitation 불필요
  • 효율이 높고 결과가 일정함
  • 정확한 downstream applications을 위해 inhibitor와 contaminant를 완벽히 제거함

제품 세부 정보

The QIAamp Virus BioRobot 9604 Kit provides automated viral nucleic acid purification on the BioRobot 9604 using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours for up to 96 samples, including bar code reading and complete process documentation, with no hands-on time during the run. 

성능

QIAamp 96 plates provide well-to-well uniformity in RNA and DNA recovery for reliable results in downstream applications. QIAamp technology yields viral RNA and DNA from cell-free body fluids ready to use in PCR and blotting procedures.

원리

No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in efficient wash steps, leaving pure nucleic acids to be eluted in a buffer provided with the kit.

절차

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use nucleic acid is then eluted in a low-salt buffer using the BioRobot 9604 (see flowchart " Protocol").

Highly pure, viral RNA and DNA can be isolated from 96 cell-free fluid samples in less than 2.5 hours. This kit can be used to isolate nucleic acids from human and animal viruses. QIAamp sample preparation technology is fully licensed.

그림 참조

응용 분야

The QIAamp Virus BioRobot 9604 Kit provides proven QIAamp technology in a convenient 96-well format for automated high-throughput purification of viral nucleic acids on the BioRobot 9604. The kit provides purification of nucleic acids from 200 µl or less of samples, including:

  • Plasma
  • Serum
  • CSF
  • Other cell-free body fluids
  • Cell-culture supernatants

지원되는 데이터 및 수치

사양

특징사양
applicationsPCR, blotting
forautomatedprocessingBioRobot 9604 Workstation
mainsampletypeSerum, plasma
elutionvolume50 µl
format96-well plates
processingAutomated
purificationoftotalrnamirnapolyamrnadnaorproteinViral DNA, viral RNA
technologySilica technology
sampleamount<200 µl
timeperrunorperprep<2.5 hours
yield>90% recovery

리소스

Kit Handbooks (1)
For simultaneous purification of viral RNA and DNA from plasma, serum, cell-culture supernatants, and cell-free body fluids using the BioRobot 9604 workstation
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Brochures & Guides (1)
Certificates of Analysis (1)

출판물

Oseltamivir-resistant influenza A 2009 H1N1 virus in immunocompromised patients.
Couturier BA; Bender JM; Schwarz MA; Pavia AT; Hanson KE; She RC;
Influenza Other Respir Viruses; 2010; 4 (4):199-204 2010 Jul PMID:20836794
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Jalal H; Bibby DF; Tang JW; Bennett J; Kyriakou C; Peggs K; Cubitt D; Brink NS; Ward KN; Tedder RS;
J Clin Microbiol; 2005; 43 (6):2575-80 2005 Jun PMID:15956366
Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods.
Yam WC; Chan KH; Chow KH; Poon LL; Lam HY; Yuen KY; Seto WH; Peiris JS;
J Clin Virol; 2005; 33 (1):19-24 2005 May PMID:15797361
A rapid method for simultaneous detection of phenotypic resistance to inhibitors of protease and reverse transcriptase in recombinant human immunodeficiency virus type 1 isolates from patients treated with antiretroviral drugs.
Hertogs K; de Béthune MP; Miller V; Ivens T; Schel P; Van Cauwenberge A; Van Den Eynde C; Van Gerwen V; Azijn H; Van Houtte M; Peeters F; Staszewski S; Conant M; Bloor S; Kemp S; Larder B; Pauwels R;
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