Bone marrow 샘플을 안정화하여 보관과 이동을 할 수 있고 intracellular RNA를 분리 정제할 수 있습니다
✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
PAXgene Bone Marrow RNA Kit (30)
카탈로그 번호 / ID. 764133
For 30 RNA preps: 30 PAXgene Spin Columns, 30 PAXgene Shredder Spin Columns, Processing Tubes, RNase-free DNase I, RNase-free reagents and buffers. To be used in conjunction with PAXgene Bone Marrow RNA Tubes
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✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
특징
- 통합된 system — storage, stabilization, and intracellular RNA purification from bone marrow
- 즉각적인 intracellular RNA stabilization으로 in vivo snapshots에 적합
- Heterogeneous bone marrow에서의 high-quality RNA의 신속한 분리 정제— cell rich sample 포함
- 안전하고 쉬운 운반 — 드라이아이스, 액체질소등 불필요
제품 세부 정보
The PAXgene Bone Marrow RNA System consists of PAXgene Bone Marrow RNA Tubes, for stabilization, storage, and transportation of bone marrow, and the PAXgene Bone Marrow RNA Kit, for silica-membrane-based RNA isolation and purification in a spin-column format. Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is immediately transferred into a PAXgene Bone Marrow RNA Tube, which stabilizes the RNA in the sample for storage at room temperature, refrigerated, or frozen. RNA can be purified days later using the PAXgene Bone Marrow RNA Kit or archived for several months at –20°C or –70°C.
성능
The PAXgene Bone Marrow RNA Kit enables efficient isolation and purification of intracellular RNA from heterogeneous human whole bone marrow samples (see figure " RNA yields from bone marrow samples"), including an optimized procedure for cell-rich, viscous samples. RNA can be purified days after collection, and intact, high-quality RNA can be purified using the PAXgene Bone Marrow RNA Kit after samples are stored at room temperature, refrigerated, or frozen (see figures " RNA stability at room temperature", "RNA stabilized at 2–8°C", and "High RNA integrity after freeze–thaw cycles"). RNA Tubes protect RNA molecules from degradation by RNases and enable accurate analysis by minimizing ex vivo changes in gene expression (see figure " Stability of 5 transcripts at room temperature"). An integrated DNase treatment ensures that genomic DNA is minimized. In-house and external studies showed that genomic DNA was <0.85% in 95% of the samples tested (n = 157). A260/A280 values are typically between 1.8 and 2.2, indicating the high purity of the RNA (see figure " Storage under different conditions").
그림 참조
원리
Copy numbers of individual mRNA species in bone marrow can change significantly during storage or transport at room temperature, making reliable studies of gene expression impossible. PAXgene Bone Marrow RNA Tubes protect RNA molecules from degradation and immediately stabilize the RNA in the sample for storage at room temperature, refrigerated, or frozen.
절차
Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is then transferred into a PAXgene Bone Marrow RNA Tube. The RNA in the sample is immediately stabilized for storage at room temperature, refrigerated, or frozen. The PAXgene Bone Marrow RNA Kit provides an easy and rapid procedure for isolation and purification of intracellular RNA from human whole bone marrow samples. The kit uses silica-membrane technology in a spin-column format for efficient purification of intact, high-quality RNA.
응용 분야
RNA purified using the PAXgene Bone Marrow RNA Kit is ready for use in a wide range of downstream applications, including
- Real-time RT-PCR analysis of specific transcripts
- Array analysis of complex expression profiles
지원되는 데이터 및 수치
Stability of 5 transcripts at room temperature.
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사양
| 특징 | 사양 |
|---|---|
| applications | RT-PCR, array analysis |
| yield | 11 µg |
| timeperrun | 95 min/12 samples |
| mainsampletype | Bone marrow samples |
| sampleamount | 2 ml |
| processing | Manual (centrifugation) |
| format | Spin column |
| elutionvolume | 80 µl (2 x 40 µl) |
| technology | Silica technology |
리소스
Brochures & Guides en (2)
Safety Data Sheets (1)
Scientific Posters (2)
Gene Expression Analysis (1)
Kit Handbooks (2)
Certificates of Analysis (1)