PAXgene Blood ccfDNA Tubes (100)
For collection of whole blood samples and stabilization of ccfDNA
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PAXgene Blood ccfDNA Tubes (100)
Cat no. / ID. 768115
100 blood collection tubes (10 ml). To be used in conjunction with the QIAamp MinElute ccfDNA Kit, the QIAamp Circulating Nucleic Acid Kit (50) or the QIAsymphony PAXgene Blood ccfDNA Kit (192)
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For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Single-tube collection, stabilization, transport and storage
- Immediate stabilization of circulating, cell-free DNA (ccfDNA)
- Sample transport and storage at room temperature for up to 10 days
- Standardized preanalytical processing of samples
Product Details
The PAXgene Blood ccfDNA Tube is a plastic, closed, evacuated tube for the collection, anticoagulation, transport and storage of human whole blood samples and the stabilization of ccfDNA. When the tube is used in conjunction with the QIAsymphony PAXgene Blood ccfDNA Kit, the QIAGEN QIAamp MinElute ccfDNA Kit or the QIAamp Circulating Nucleic Acid Kit, high-quality ccfDNA can be isolated for use in downstream analytical assays.
Performance
PAXgene Blood ccfDNA Tubes simplify the collection and processing of whole blood for subsequent purification of circulating, cell-free DNA (ccfDNA). The tube additive stabilizes blood cells (see " PAXgene Blood ccfDNA stabilization helps prevent hemolysis allowing distinct separation of plasma" and " PAXgene Blood ccfDNA stabilization minimizes hemolysis compared to alternative solutions") and blocks apoptosis (see " PAXgene Blood ccfDNA stabilization helps prevent apoptosis and fragmentation of gDNA"). Consequently, using PAXgene Blood ccfDNA Tubes for whole blood collection, storage and transport helps prevent the release of intracellular DNA into plasma (see " PAXgene Blood ccfDNA stabilization helps prevent release of gDNA into plasma"). ccfDNA levels are stable for up to 10 days at temperatures up to 25°C, 7 days at temperatures up to 30°C, or 3 days at temperatures up to 37°C prior to processing, so blood samples can be transported and stored to process plasma later.
Plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes and processed with the QIAsymphony PAXgene Blood ccfDNA Kit, the QIAGEN QIAamp MinElute ccfDNA Kit or the QIAamp Circulating Nucleic Acid Kit, yields high-quality ccfDNA for downstream analytical assays, including but not limited to PCR-based assays and next-generation sequencing (NGS).
See figures
PAXgene Blood ccfDNA stabilization reagent helps prevent hemolysis, allowing clear distinction between plasma and cell fractions.
PAXgene Blood ccfDNA stabilization minimizes hemolysis compared to alternative solutions.
PAXgene Blood ccfDNA stabilization helps prevent apoptosis and fragmentation of gDNA in whole blood.
PAXgene Blood ccfDNA stabilization reagent helps prevent release of gDNA into plasma.
PAXgene Blood ccfDNA stabilization minimizes hemolysis compared to alternative solutions.
PAXgene Blood ccfDNA stabilization helps prevent apoptosis and fragmentation of gDNA in whole blood.
PAXgene Blood ccfDNA stabilization reagent helps prevent release of gDNA into plasma.
Principle
The PAXgene Blood ccfDNA Tube is a plastic, closed, evacuated tube for the collection, anticoagulation, transport and storage of human whole blood samples and the stabilization of ccfDNA. The tube contains an additive that anticoagulates blood and stabilizes blood cells via a non-crosslinking stabilization solution. This helps prevent the release of intracellular DNA into the plasma and maintains constant ccfDNA levels during sample storage before processing.
Procedure
The PAXgene Blood ccfDNA Tube allows collection of 10 ml whole blood into a closed, evacuated system. Using standard venipuncture technique, blood is collected into an evacuated tube that contains 1.5 ml cell stabilization additive, resulting in a ratio of 0.15 ml additive per 1 ml blood. Isolation of ccfDNA from plasma is carried out using the QIAsymphony PAXgene Blood ccfDNA Kit, the QIAamp MinElute ccfDNA Kit or the QIAamp Circulating Nucleic Acid Kit.
Applications
ccfDNA purified from plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes can be used in a wide range of downstream applications, including:
- PCR, including digital, multiplex and quantitative real-time PCR
- Pharmacogenomic studies
- SNP genotyping
- Next-generation sequencing
Supporting data and figures
PAXgene Blood ccfDNA stabilization reagent helps prevent release of gDNA into plasma.
Whole blood was stored in EDTA tubes or PAXgene Blood ccfDNA Tubes. ccfDNA was extracted from the plasma immediately following blood collection (t0) or after 6 days storage (t6) at room temperature. Eluate (1 µl) was analyzed using the Agilent High Sensitivity DNA Kit. Plasma from EDTA tubes showed an increase in apoptotic gDNA fragments, whereas plasma from PAXgene Blood ccfDNA Tubes showed a ccfDNA profile comparable to day 0.
Specifications
| Features | Specifications |
|---|---|
| tubesize | 16 x 100 mm |
| stabilization | Up to 10 days at temperatures up to 25°C, 7 days at temperatures up to 30°C, or 3 days at temperatures up to 37°C. Note: Do not store blood-filled tubes below 2°C. |
| drawvolume | 10 ml |
| labeltype | Paper |
| quantity | 100 tubes/case |
| closuretypeandcolor | Pearl white BD Hemogard/Blue stopper |
| additiveandconcentration | Additive 1.5 ml |
Resources
Brochures & Guides (1)
Scientific Posters (10)
Safety Data Sheets (1)
Brochures & Guides en (1)
Kit Handbooks (1)
Application Notes (2)
Supplementary Protocols (1)
Certificates of Analysis (1)