QIAcuity Nanoplates y accesorios

Para uso con los instrumentos de PCR digital QIAcuity

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QIAcuity Nanoplate 26k 8-well (10)

N.º de cat. / ID. 250031

10 QIAcuity Nanoplate 26k 8-well, 11 Nanoplate Seals

6621,00 MXN

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Product TypeAccessories

QIAcuity Nanoplate

Nanoplate Seals

Nanoplate Tray

Nanoplate Adapter

Particiones por pocillo

26k

8.5k

Número de pocillos

Cantidad

QIAcuity Nanoplates y accesorios están concebidos para su uso en aplicaciones de biología molecular. Estos productos no están concebidos para el diagnóstico, la prevención ni el tratamiento de enfermedades.

✓ Procesamiento automático sin interrupción de pedidos en línea

✓ Servicio técnico y para productos experto y profesional

✓ Realización y repetición de pedidos rápidas y fiables

Características

Cuatro nanoplacas para diferentes necesidades de aplicación
Hasta 8500 o 26 000 divisiones por pocillo
Formato SBS

Detalles del producto

Las QIAcuity Nanoplates son placas de PCR digital de microfluidos que permiten analizar 8, 24 o 96 muestras con un máximo de 8500 o 26 000 divisiones por pocillo. Las cuatro nanoplacas están diseñadas para utilizarse en los instrumentos de dPCR QIAcuity.

Las nanoplacas solo pueden utilizarse con el QIAcuity Digital PCR System. Use el QIAcuity Nanoplate Adapter dedicado al llevar a cabo la configuración automatizada de PCR y manipulación de líquido en una QIAcuity Nanoplate con el sistema QIAgility. Una vez hecho esto, cargue la placa en el QIAcuity Digital PCR System para la reacción de dPCR.

¿Le gustaría obtener más información sobre el producto y que uno de nuestros especialistas en dPCR se ponga en contacto con usted? Regístrese aquí y nos pondremos en contacto con usted en breve.

Rendimiento

Estas placas están especialmente diseñadas para utilizarse con reacciones de PCR digital en los instrumentos QIAcuity. QIAGEN ofrece cuatro tipos de nanoplacas, todas con formato SBS, pero con diferentes especificaciones para distintas necesidades de aplicación.

Características y especificaciones de las nanoplacas
Tipo	Color del bastidor	Especificaciones	Aplicaciones
Nanoplate 26K 8-well	Azul claro	8 pocillos × aprox. 26 000 divisiones Reacción de dPCR de 40 µl por pocillo	Detección de mutaciones poco frecuentes, biopsia líquida, análisis de expresión génica, detección de microrganismos patógenos, etc.
Nanoplate 26K 24-well	Azul	24 pocillos × aprox. 26 000 divisiones Reacción de dPCR de 40 µl por pocillo	Detección de mutaciones poco frecuentes, biopsia líquida, análisis de expresión génica, detección de microrganismos patógenos, etc.
Nanoplate 8.5K 24-well	Blanco	24 pocillos × aprox. 8500 divisiones Reacción de dPCR de 12 µl por pocillo	Análisis de variación en el número de copias, análisis de expresión génica, cuantificación de la genoteca de NGS, detección de modificaciones del genomas, etc.
Nanoplate 8.5K 96-well	Gris	96 pocillos × aprox. 8500 divisiones Reacción de dPCR de 12 µl por pocillo

Principio

En solo 3 simples pasos, puede obtener el resultado de dPCR que desea en menos de dos horas: pipetee y cargue, realice el experimento y analice los resultados. El principio de la reacción de dPCR en las nanoplacas se describe aquí.

Procedimiento

Al igual que en los experimentos de qPCR, la preparación de las muestras incluye la transferencia de mezcla maestra, sondas y cebadores a una nanoplaca de 8, 24 o 96 pocillos, seguida de la adición de las muestras. El sistema integra funciones de división, termociclado y obtención de imágenes en un solo instrumento completamente automatizado que permite a los usuarios obtener los resultados de las muestras en menos de 2 horas. Es posible realizar un análisis en el paquete de software, que proporciona la concentración en copias por microlitro de la secuencia de blancos y también para el control de calidad como muestras positivas o NTC. Este análisis también se puede extender a ordenadores remotos dentro de la misma red de área local (LAN).

Aplicaciones

Las QIAcuity Nanoplates, junto con el QIAcuity Digital PCR System y los QIAcuity PCR kits posibilitan las aplicaciones de PCR digital, entre las que se incluyen:

Detección de mutaciones poco frecuentes
Análisis de variación en el número de copias
Análisis de expresión génica
Detección de microrganismos patógenos
Genotipado
Investigación sobre miARN
Terapia celular y génica
Cuantificación del ADN residual
Control de aguas residuales

Recursos

Version 1.0

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.

This new version of the control software includes system stability and performance reliability improvements. We highly recommend to update the control software of your QIAcuity instrument.

Note: The installation requires approximately 90 minutes.

Version 2.0

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis

Besides system stability and performance reliability improvements, this new version of the control software offers advanced user management and audit trail functionalities. Together with the QIAcuity Software Suite 2.0, the QIAcuity systems now support 21 CFR Part 11 regulations for users working under GMP.

Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.

Note: Upgrading the QIAcuity Control Software to version 2.0 will require upgrading the QIAcuity Software Suite to version 2.0.

Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.0. It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!

Version 2.1

Beside of improvements and bug fixes, the new features mainly address the GMP requirements by offering additional support for 21 CFR part 11 compliance and get the software ready for future Nanoplate support.

For more information, please refer to the Release Note: QIAcuity Instrument Control Software, Version 2.1.

SHA1 checksum: 0DD41A8DE46D10E81F538ACB71AC184170D39FE0

Version 1.1.3

Version 1.2

The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

The following browsers are supported in the QIAcuity Software Suite:

-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)

The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.

Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.

The new improvements are as follows:

-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting

Version 4.0

The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.

After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.

Required QIAcuity Software Suite version: Version 1.2 or higher.

Version 2.0

The QIAcuity Software Suite 2.0 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

This new software version now supports 21 CFR Part 11 regulations for users working under GMP. This includes the following:

-Advanced User Management
-Audit Trail
-Signatures

Additional changes compared with the previous software version include the following:

-Usability improvements
-Improved dust detection
-Hyperwell expansion to CNV and GEX analysis
-CSV export functionality of multiple positive partitions for linkage analysis, drop-off assays, etc.
-Raw data export

Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.

Important: A direct update of the Software Suite from version 1.1.3 to 2.0.20 is not possible. Please follow the instructions in the User Manual on how to update to version 1.2.18 first before updating the Software Suite to version 2.0.20.

Before you start upgrading to version 1.1.3, please make sure that all plates are imported and visible in the plate overview of the QIAcuity Software Suite.

Caution: Not following the instructions may result in a loss of your previous plate data!

Please contact QIAGEN Technical Services if you are unsure and require technical support.

SHA1 checksum: C60C4EEC349DF1C037796016D32A93EEC19B54A0

Version 2.1

Beside of usability improvements and bug fixes, the new features address GMP requirements by offering additional support for 21 CFR part 11 compliance, copy number variation (CNV) analysis, support of future Nanoplates, and improved 1D scatterplot views.

For more information, please refer to the Release Note: QIAcuity Software Suite, Version 2.1

SHA1 checksum: 6D51E273B26FA7D7E0EDA843CB3DCD74B38FA5B1

For Version 1.0

For Version 1.2

For Version 2.0

For Version 2

Version 2.1

This study tested a workflow for quantitation and qualification of AAV samples using a duplex assay on the QIAcuity dPCR instrument targeting both an insert (GFP) and the viral backbone (AAV2-ITR). With very low intra-assay and inter-assay CVs <6.5%, we demonstrate one of the main benefits of dPCR: reproducibility.

Here we report the use of saliva samples in combination with dPCR as a suitable alternative to screen for individuals infected with SARS-CoV-2.

Digital PCR is a superior method to qPCR for the detection and absolute quantification of low concentration target templates. There are multiple digital PCR systems on the market that differ in numerous aspects including the amount of dead volume, which is the volume that is loaded but not analyzed by the given instrument. While it has been speculated that dead volume could impact the sensitivity of dPCR applications, here we provide data to support the conclusion that the most important factors in determining the relative sensitivity of each system are template addition volume and template analyzed volume. In summary, data provided herein demonstrate that higher template addition volumes can overcome any limitations that dead volume may have on the sensitivity of a dPCR application.

The QIAgility instrument is a liquid handler designed for automating PCR setup. For compatibility with the QIAcuity, we developed an adapter to secure up to two nanoplates onto the deck of the QIAgility. Using the QIAgility software, we have optimized a protocol that works for all nanoplate types and QIAcuity applications. Here we report the performance of a front end automated QIAgility dPCR nanoplate setup procedure for use with the QIAcuity dPCR system.

The QIAcuity digital PCR system combined with the QIAcuity One-Step Viral RT-PCR Kit enables precise detection and quantitation of vector-borne viruses in mosquitoes. The results presented in this comparison study showed that digital PCR is a powerful tool for absolute quantitation of low abundant targets and is a more reliable detection method than qPCR. Multiplexing allows detection and quantitation of multiple targets in a single reaction more efficiently by increasing sample throughput at a reduced cost per target.

The goal of this work was to compare performance of quantitative PCR (qPCR) and digital PCR (dPCR) in the quantification of gene expression and Wolbachia abundances in Nasonia parasitoid wasps.

Here we provide an integrated rAAV genome titer method using the QIAcuity Digital PCR (dPCR) System with detailed parameters for high assay performance. Using this optimized method for pre-PCR handling of in-process rAAV samples, the results demonstrated that QIAcuity dPCR system generates the same level of accuracy and precision as the current gold standard ddPCR system but with much faster sample-to-result times (2 hours vs 7 hours) and higher overall throughput and scalability.

Here we compared the performance of qPCR and the nanoplate dPCR techniques. The digital PCR method on the QIAcuity significantly improved precision when measuring copy number states and sensitivity of mutation detection through absolute quantification and reduced standard error. This is advantageous in various applications, including copy number variation analysis, small fold-change and rare mutation detection.

Here we demonstrate how to optimize your assays on a microfluidic nanoplate-based digital PCR system, the QIAcuity, and provide recommendations for a seamless transfer. Moreover, the QIAcuity dPCR workflow is very similar to qPCR.

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For use with QIAcuity systems

July 2021

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QIAcuity Nanoplates y accesorios

Para uso con los instrumentos de PCR digital QIAcuity

QIAcuity Nanoplate 26k 8-well (10)

Características

Detalles del producto

Rendimiento

Principio

Procedimiento

Aplicaciones

Recursos