Reaction당 50 ng부터 2 ug까지의 RNA를 reverse transcription을 할 수 있습니다
상업적인 목적을 위해 제품이 대량으로 필요하거나 맞춤형 또는 최적화 제품이 필요하십니까? QIAGEN에서는 물류, 규정 준수 등에 대한 지원도 제공합니다. QIAGEN에 문의하여 전략적 파트너십과 OEM을 통해 협력하는 방법을 알아보세요
Omniscript RT Kit (200)
카탈로그 번호 / ID. 205113
For 200 reverse-transcription reactions: 800 units Omniscript Reverse Transcriptase, 4 x 150 µl 10x Buffer RT, 4 x 100 µl dNTP Mix (contains 5 mM each dNTP), 4 x 1.1 ml RNase-free water
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200
50
Omniscript RT Kit은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
상업적인 목적을 위해 제품이 대량으로 필요하거나 맞춤형 또는 최적화 제품이 필요하십니까? QIAGEN에서는 물류, 규정 준수 등에 대한 지원도 제공합니다. QIAGEN에 문의하여 전략적 파트너십과 OEM을 통해 협력하는 방법을 알아보세요
특징
- High yields of cDNA
- 특이성을 높인 참신한 enzyme과 buffer
- No additional RNase H digestion step required
- Robust system guarantees consistent results
제품 세부 정보
The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low-copy numbers. The Omniscript RT Kit includes Omniscript Reverse Transcriptase, dNTPs and an optimized reaction buffer. Simply add primers for fast and easy cDNA synthesis.
성능
The high affinity of Omniscript Reverse Transcriptase (RT) results in highly specific and sensitive RT-PCR, even with low-copy transcripts (see figure " Sensitive RT-PCR of ≥10 copies"), and the ability to read through even complex RNA secondary structures without adjusting temperature or reaction conditions (see figure " Comparison of various reverse transcriptases").
Regions of RNA with high GC content can cause other reverse transcriptases to stop, dissociate from the RNA template, or skip over looped-out regions of RNA (see figure " Full-length RT-PCR products — B"). These difficult templates, however, prove no problem for QIAGEN reverse transcriptases (see figure "F ull-length RT-PCR products — A"). With no need for optimization, the Omniscript RT Kit makes reverse transcription virtually trouble-free.
그림 참조
원리
Omniscript Reverse Transcriptase (RT) has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, leading to high yields of cDNA. It is provided ready-to-use with dNTPs and in an optimized reaction buffer that, together with the high affinity of Omniscript RT for RNA, enables read-through of templates with high GC content or complex secondary structure. Please note that the primer mix is not provided.
Omniscript RT is specially designed for all reverse transcription with any amount of RNA from 50 ng to 2 µg per reaction. Omniscript RT is also usually the enzyme of choice with viral RNA due to the presence of carrier RNA in most viral RNA preparations. In comparative experiments, Omniscript RT consistently outperforms other reverse transcriptases over a wide range of starting RNA amounts.
Lot-to-lot reproducibility of Omniscript Kits is ensured by rigorous quality control at QIAGEN. The optimized Buffer RT, dNTPs, and water included in all Omniscript RT Kits are guaranteed RNase-free, and each lot of Omniscript RT is thoroughly tested for RT-PCR reproducibility.
절차
With the optimized Omniscript Reverse Transcriptase reaction buffer, tedious pipetting and pre-incubation steps are eliminated, and no additional RNase H digestion step is needed (see flowchart " Omniscript Reverse Transcriptase Procedure").
그림 참조
응용 분야
Omniscript Reverse Transcriptase is suitable for use in the following applications:
- cDNA synthesis for end-point PCR
- Synthesis of double-stranded cDNA for cloning
- RACE (Rapid Amplification of cDNA Ends)
- Linear RNA amplification
- Exponential RNA amplification
- SAGE (Serial Analysis of Gene Expression)
- Labeling for microarrays
- Analysis of transcription start site by primer extension
지원되는 데이터 및 수치
Full-length RT-PCR products — A.
RT-PCR was carried out with total RNA from maize leaves using the indicated RT reaction temperatures. A 1.2 kb fragment of the GC-rich maize gl2 transcript was amplified. RT was carried out using the standard Omniscript RT protocol at the standard (37°C) and higher reaction temperatures as indicated.
사양
| 특징 | 사양 |
|---|---|
| applications | RT-PCR, qRT-PCR, primer-extension, RACE analysis |
| realtimeorendpoint | Endpoint |
| mastermix | No |
| enzymeactivity | Reverse transcription |
| singleormultiplex | Single |
| withwithouthotstart | Without hotstart |
| reactiontype | Reverse transcription |
| sampletargettype | RNA template |
리소스
Quick-Start Protocols (1)
Safety Data Sheets (1)
Kit Handbooks (1)
Application Notes (1)
Certificates of Analysis (1)