RT2 SYBR® Green FAST Mastermixes
用于real-time PCR分析
Products
RT2 SYBR® Green FAST Mastermixes 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。
RT² SYBR Green ROX FAST Mastermix (2)
Cat. No. / ID. / ID. 330620
Contains 2 x 1.35 ml tubes: for 12 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 250 x 20 µl reactions
RT² SYBR Green FAST Mastermix (8)
Cat. No. / ID. / ID. 330601
Contains 8 x 1.35 ml tubes: for 8 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1000 x 20 µl reactions
RT² SYBR Green ROX FAST Mastermix (24)
Cat. No. / ID. / ID. 330623
Contains 24 x 1.35 ml tubes: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 3000 x 20 µl reactions
RT² SYBR Green ROX FAST Mastermix (25 ml)
Cat. No. / ID. / ID. 330629
Contains 1 bottle of 25 ml mastermix: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 2500 x 20 µl reactions
RT² SYBR Green FAST Mastermix (12)
Cat. No. / ID. / ID. 330602
Contains 12 x 1.35 ml tubes: for 12 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1500 x 20 µl reactions
RT² SYBR Green ROX FAST Mastermix (8)
Cat. No. / ID. / ID. 330621
Contains 8 x 1.35 ml tubes: for 8 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1000 x 20 µl reactions
RT² SYBR Green FAST Mastermix (2)
Cat. No. / ID. / ID. 330600
Contains 2 x 1.35 ml tubes: for 2 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 250 x 20 µl reactions
RT² SYBR Green ROX FAST Mastermix (12)
Cat. No. / ID. / ID. 330622
Contains 12 x 1.35 ml tubes: for 12 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 1500 x 20 µl reactions
RT² SYBR Green FAST Mastermix (24)
Cat. No. / ID. / ID. 330603
Contains 24 x 1.35 ml tubes: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 3000 x 20 µl reactions
特点
- 特异性扩增，引物二聚体较少
- 一体化混合物，适用于特定仪器
- 可选含有ROX、荧光素或不含染料的规格
- 实验步骤比常规SYBR Green预混液的步骤少
- 灵敏度高、解离曲线好
产品详情
RT2 SYBR Green FAST Mastermixes非常适合使用SYBR Green进行real-time PCR分析，可选含有或不含参比染料的规格。RT2 SYBR Green FAST Mastermixes经优化后具有更快的PCR操作流程，而不影响灵敏度和特异性。RT2 SYBR Green FAST Mastermixes应与RT2 qPCR Primer Assays和RT2 Profiler PCR Arrays配合使用。
绩效
RT² SYBR Green FAST Mastermixes包含所有优化的试剂和缓冲液，可在各种real-time PCR分析仪上配合RT2 qPCR Primer Assays和RT2 Profiler PCR Arrays进行SYBR Green real-time PCR分析。
RT² SYBR Green FAST Mastermixes进行的高性能real-time PCR具有较高的扩增效率（参见" High amplification efficiency over a wide dynamic range"）和高灵敏度及特异性（参见" Tighter control of polymerase activity yields greater specificity"）。
此外，与常规方法获得的分离曲线相比，使用RT2 SYBR Green FAST Mastermixes得到的分离曲线有更低的背景和更明显的曲线（参见" Sharper dissociation curves with RT2 SYBR Green Fast Mastermix"）。
查看图表
原理
RT2 SYBR Green FAST Mastermixes包含real-time PCR缓冲液、高性能的HotStart DNA Taq聚合酶、核苷酸和SYBR Green染料。有些预混液包含fluorescein荧光素或ROX染料，用于优化real-time PCR分析仪的光学元件。化学修饰并严格控制的HotStart酶可避免扩增引物二聚体和其他非特异性产物，提供准确的SYBR Green结果。RT2 SYBR Green FAST Mastermixes经优化后使用10s变性和30s退火/延伸的PCR实验方案，而不影响特异性和灵敏度。
不含ROX或荧光素的预混液
RT2 SYBR Green FAST Mastermix非常适合在不需要参比染料的仪器上进行基于SYBR Green的real-time PCR应用，仪器包括Bio-Rad models CFX96、CFX384；Bio-Rad/MJ Research Chromo4、DNA Engine Opticon、DNA Engine Opticon 2；Roche LightCycler 480（96孔和384孔）；无ROX滤过装置的Eppendorf Mastercycler ep realplex以及Cepheid SmartCycler。
含有荧光素的预混液
RT2 SYBR Green Fluor FAST Mastermix非常适合在需要fluorescein荧光素作为参比染料的仪器上进行基于SYBR Green的real-time PCR应用，仪器包括Bio-Rad models iCycler、iQ5、MyiQ和MyiQ2。
含有ROX的预混液
RT2 SYBR Green ROX FAST Mastermix非常适合在需要ROX作为参比染料的仪器上进行基于SYBR Green的real-time PCR应用，仪器包括QIAGEN的Rotor-Gene Q实时荧光定量PCR分析仪；Applied Biosystems models 5700、7000、7300、7500（标准和快速）、7700、7900HT（标准和快速的96-well block，384-well block）、StepOnePlus和ViiA 7（标准和快速的96-well block，384-well block）；含有或无ROX滤过装置的Eppendorf Mastercycler ep realplex；Stratagene models Mx3000P、Mx3005P、Mx4000以及Takara TP-800。
应用
RT2 SYBR Green FAST Mastermixes适合用于real-time PCR分析。
辅助数据和图表
Sharper dissociation curves with RT2 SYBR® Green FAST Mastermix.
A positive PCR control was amplified on the ABI 7500 qRT-PCR instrument using [A] regular cycling and RT² SYBR Green Mastermix or [B] fast cycling with RT² SYBR Green FAST Mastermix. The dissociation curve obtained with RT² SYBR Green FAST Mastermix has lower background and a sharper shape.
资源
安全数据表 (1)
试剂盒操作手册 (1)
Certificates of Analysis (1)