Plants와 fungi에서 total RNA 100 ug까지 분리 정제할 수 있습니다
✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
RNeasy Plant Mini Kit (50)
카탈로그 번호 / ID. 74904
50 RNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
로그인 계정 가격 보기.
RNeasy Plant Mini Kit은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
특징
- 30 분 안에 양질의 RNA 추출
- No phenol/chloroform extraction
- CsCl gradients, LiCl 또는 ethanol precipitation 불필요
- 뛰어난 RNA recovery
- Ready-to-use RNA for any downstream application
제품 세부 정보
The RNeasy Plant Mini Kit includes QIAshredder spin columns for homogenizing and filtering viscous plant or fungal lysates, and RNeasy spin columns for purifying up to 100 μg of high-quality RNA using silica-membrane technology. Purification can be fully automated on the QIAcube. The kit can also be used in combination with a TissueRuptor or TissueLyser system, which provide efficient disruption and homogenization of plant samples.
성능
The RNeasy Plant Mini Kit is ideal for isolation of total RNA from a wide variety of plant and fungal samples with sample sizes of 10–100 mg tissue, or 100–1 x 107 cells (see table "Selected samples processed with the RNeasy Plant Mini Kit"). The binding capacity of the spin-columns is up to 100 µg RNA. The typical yield from 100 mg plant tissue is between 25 µg and 65 µg of RNA, although amounts of RNA can vary due to the developmental stage and growth conditions of the samples (see table "Yield from 100 mg tissue").
| Plants | Filamentous fungi |
|---|---|
| Anemone sp. Arabidopsis thaliana1 Begonia sp. Beta vulgaris (sugar beet)2 Chlamydomonas reinhardii (unicellular alga) Chrysanthemum Clarkia spp.3 Daucus carota (carrot)4 Diascia sp. Dendranthema sp. Euglena gracilis (unicellular alga) Funaria hygrometrica (moss) Hordeum vulgare (barley) Lycopersicon esculentum (tomato)5 Malus sp. (apple)6 Nicotiana tabacum (tobacco) Oryza sativa (rice) Pelargonium sp. (geranium) Petroselinum crispum (parsley)7 Pisum sativum (pea) Prunus sp. (cherry)6 Ranunculus sp. Ribes nigrum (black currant) Riccia fluitans (liverwort) Sinapis arvensis (mustard) Solanum tuberosum (potato)8 Spinacia oleracea (spinach) Surfinia sp. Triticum aestivum (wheat) Vitis sp. (grapevine)6 Zea mays (maize) | Acremonium crysogenum9 Fusarium avenaceum9 |
| Sample type | Yield |
|---|---|
| Arabidopsis leaves | 35 µg |
| Maize leaves | 25 µg |
| Tomato leaves | 65 µg |
| Tobacco leaves | 60 µg |
The purified plant RNA, eluted in 30–100 µl volume, includes viral RNA (see figure " Detection of viruses"). All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications (see figure " Tissue specificity of histone H4 expression").
그림 참조
원리
The RNeasy Plant Mini Kit provides QIAshredder columns for homogenization and filtration of viscous plant or fungal lysates by microcentrifugation in combination with RNeasy Mini spin columns for RNA purification. RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification. RNeasy Kits provide the highest-quality RNA with minimum copurification of DNA. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using convenient on-column DNase treatment during the RNeasy procedure.
절차
Samples are first lysed and then homogenized in the QIAshredder columns. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy silica membrane. RNA binds, and all contaminants are efficiently washed away. Pure, concentrated RNA is eluted in water (see flowchart " RNeasy Plant Mini procedure").
그림 참조
응용 분야
RNA purified with RNeasy technology is high-quality and is ideal for use in all applications. Downstream applications include:
- Northern, dot, and slot blotting
- End-point RT-PCR
- Quantitative, real-time RT-PCR
- Array analysis
- Poly A+ RNA selection
지원되는 데이터 및 수치
RNeasy Plant Mini procedure.
RNeasy Plant Mini procedure.
사양
| 특징 | 사양 |
|---|---|
| applications | PCR, qPCR, real-time RT-PCR, microarray |
| elutionvolume | 30–100 µl |
| sampleamount | 10–100 mg |
| processing | Manual |
| mainsampletype | Plant samples |
| purificationoftotalrnamirnapolyamrnadnaorprotein | RNA |
| format | Spin column |
| technology | Silica technology |
| yield | 25–60 µg |
| timeperrunorperprep | 30 minutes |
리소스
Safety Data Sheets (1)
Brochures & Guides en (1)
Quick-Start Protocols (1)
User-Developed Protocols (2)
Supplementary Protocols (1)
Gene Expression Analysis (1)
Certificates of Analysis (1)
출판물
Coordination of microbe-host homeostasis by crosstalk with plant innate immunity.
Nat Plants; 2021; 7 (6):814-825 2021 May 24 PMID:34031541
RAF-like protein kinases mediate a deeply conserved, rapid auxin response.
Cell; 2023; 187 (1):130-148.e17 2023 Dec 20 PMID:38128538
The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase.
Nat Genet; 2023; 55 (6):921-926 2023 May 22 PMID:37217714
Cell-cycle-linked growth reprogramming encodes developmental time into leaf morphogenesis.
Curr Biol; 2024; 34 (3):541-556.e15 2024 Jan 19 PMID:38244542
Wheat breeding history reveals synergistic selection of pleiotropic genomic sites for plant architecture and grain yield.
Mol Plant; 2022; 15 (3):504-519 2022 Jan 10 PMID:35026438