Products
QIAsymphony DNA Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Features
- Purification of DNA from a broad range of sample types
- Novel, prefilled reagent cartridges for ease of use
- High reproducibility through standardized processing
- Bar codes enable tracking of samples and reagents
Product Details
QIAsymphony DNA Kits enable automated purification of DNA from a broad range of sample types on the QIAsymphony SP with sample volumes of up to 1 ml. Genomic DNA can be purified from human whole blood, buffy coat, human and animal tissues, formalin-fixed paraffin-embedded tissue (FFPE), cultured cells, and bacterial cultures. The QIAsymphony DNA Mini Kit also enables purification of viral nucleic acids from human whole blood. Optimized protocols provide high yields of pure DNA, and flexible elution volumes allow DNA concentration to be easily optimized for each downstream application. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
Performance
Automated purification of DNA on the QIAsymphony SP provides high yields of DNA (see figure " High yields of DNA from human whole blood"). Spectrophotometric analysis showed that the DNA was highly pure (see figure " Highly pure DNA"). DNA gave high-performance results in downstream PCR of a single-copy gene with no inhibition of PCR observed with 1 µl or 10 µl eluate in the amplification reaction (see figures " High-performance results in PCR — A" and " High-performance results in PCR — B").
See figures
Principle
QIAsymphony DNA Kits combine the speed and efficiency of silica-based purification of genomic DNA with the convenient handling of magnetic particles (see figure " Efficient processing of magnetic particles"). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.
See figures
Procedure
For tissues, cultured cells, and bacteria protocols, manual sample pretreatment is required. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different elution volumes depending on the protocol. The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples are processed in a single run.
| Protocol | Kit | Sample material | Processed volume |
|---|---|---|---|
| DNA Blood 200 | QIAsymphony DNA Mini Kit (192) | Human whole blood | 200 μl |
| DNA Blood 400 | QIAsymphony DNA Midi Kit (96) | Human whole blood | 400 μl |
| DNA Blood 1000 | QIAsymphony DNA Midi Kit (96) | Human whole blood | 1000 μl |
| DNA Buffy Coat 200 | QIAsymphony DNA Mini Kit (192) | Buffy coat | 200 μl |
| DNA Buffy Coat 400 | QIAsymphony DNA Midi Kit (96) | Buffy coat | 400 μl |
| Tissue LC 200 | QIAsymphony DNA Mini Kit (192) | Pretreated sample lysates from tissue, FFPE tissue, cultured cells, and bacterial cultures; for example: heart (25 mg), lung (25 mg), kidney (25 mg), muscle (50 mg), Jurkat cells (2 x 106cells)* | 200 μl |
| Tissue HC 200 | QIAsymphony DNA Mini Kit (192) | Pretreated sample lysates from tissue, cultured cells, and bacterial cultures; for example: spleen (25 mg), liver (25 mg), Jurkat cells (1 x107 cells), bacteria (4 x 109 cells)* | 200 μl |
| Virus Blood 200 | QIAsymphony DNA Mini Kit (192) | Human whole blood | 200 μl |
Applications
DNA purified using QIAsymphony DNA Kits is of high quality and is ready for use in downstream applications, such as:
- PCR and real-time PCR
- Genotyping, STR, and SNP analysis
- Sequencing
Supporting data and figures
High yields of DNA from human whole blood.
Total DNA was purified from human whole blood (3 donors, 6 replicates each) on the QIAsymphony SP using the QIAsymphony DNA Mini Kit (200 µl) or QIAsymphony DNA Midi Kit (1000 µl). Blood was collected in BD Vacutainer K2E tubes and stored at 5–8°C prior to use. Yield was determined by spectrophotometric analysis.
Specifications
| Features | Specifications |
|---|---|
| applications | PCR, Real-Time PCR, Genotyping |
| sampletype | Blood; Cell culture (only Mini Kit); Tissue (only Mini Kit) |
| elutionvolume | Mini Kit: 50-200 µl; Midi Kit: 100-500 µl |
| sampleamount | Mini Kit: 200 µl blood, up to 50 mg tissue or up to 1 x 10e7 cells; Midi Kit: 400 µl or 1000 µl blood |
| yield | Mini Kit: 4-10 µg from 200 µl blood; Midi Kit: 8-20 µg from 400 µl blood or 15-50 µg from 1000 µl blood |
Resources
Application/Protocol Documents (7)
Safety Data Sheets (1)
Scientific Posters (1)
Labware Files and Documents (1)
Kit Handbooks (2)
Certificates of Analysis (1)