QIAsymphony PAXgene Blood RNA Kit
For automated purification of intracellular RNA (including miRNAs) from stabilized blood
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QIAsymphony PAXgene Blood RNA Kit (96)
Cat no. / ID. 762635
For 96 preps: 2 Reagent Cartridges, Enzyme Racks, accessories, and RNase-free buffers
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For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Want to try this solution for the first time?
Get in touch with our specialists if you need a quote or if you would like to discuss your project.
Features
- Improved RNA quality and yield
- Effective copurification of miRNA
- Prefilled reagent cartridges for safety and ease of use
- High reproducibility through standardized processing
- Bar codes enable tracking of samples and reagents
Product Details
The QIAsymphony PAXgene Blood RNA Kit provides fully automated purification of cellular RNA on the QIAsymphony SP. The new, improved kit provides higher-quality RNA, including miRNA. Used with PAXgene Blood RNA Tubes (available from BD and BD authorized distributors, cat. no. 762165) for sample collection and stabilization, the system provides a complete preanalytical solution, from sample collection and stabilization through to purification of high-quality RNA using proven magnetic particle technology.
Performance
The fully automated PAXgene Blood RNA procedure provides RNA yields comparable to the manual PAXgene Blood RNA procedure. Typical yields of RNA isolated from 2.5 ml healthy human whole blood (4.6 x 106 - 1.1 x 107 leukocytes/ml) are ≥3 μg for >95% of the samples processed with all PAXgene Blood RNA Kits. The automated PAXgene Blood RNA procedure provides high-quality RNA from different donor samples with enrichment of small RNA species (see figure " High-quality RNA from different donors"). Since yields are highly donor-dependent, individual yields may vary. The integrated DNase treatment ensures that genomic DNA contamination is minimized. Purified RNA is ready to use in downstream applications, such as quantitative, real-time RT-PCR.
See figures
Principle
The QIAsymphony PAXgene Blood RNA Kit provides fully automated purification of intracellular RNA on the QIAsymphony SP. Used with PAXgene Blood RNA Tubes (available from BD and BD authorized distributors, cat. no. 762165) for sample collection and stabilization, the system provides a complete preanalytical solution from sample collection and stabilization through to purification of high-quality RNA using proven magnetic-particle technology. Prefilled cartridges reduce manual handling to a minimum, and bar code labeling enables full reagent tracking.
Procedure
The QIAsymphony PAXgene Blood RNA procedure begins with collection of blood samples (2.5 ml) in PAXgene Blood RNA Tubes. Samples can be stored or transported at room temperature. The purification procedure starts with a centrifugation step to pellet the contents of each PAXgene Blood RNA Tube. After resuspension of the pellet, the tubes are transferred to the QIAsymphony SP. Loading of the reagent cartridge and plasticware is guided and checked by dedicated software. The QIAsymphony SP then carries out the fully automated RNA purification procedure. An integrated DNase treatment ensures that genomic DNA contamination is minimized (see figure " QIAsymphony PAXgene Blood RNA procedure"). The robust and standardized procedure together with on-board measures of the QIAsymphony SP is designed to minimize the risk of sample-to-sample cross-contamination (see figure " On-board measures against cross-contamination"). After purification, a final heat treatment of the eluate enhances performance in downstream applications. Total processing time is about 2.5 hours for 24 samples, which includes a maximum of 25–30 minutes of hands-on time.
See figures
Applications
RNA purified using the QIAsymphony PAXgene Blood RNA Kit and the QIAsymphony SP is highly pure and ready for use in a wide range of sensitive downstream applications, including:
- RT-PCR and quantitative, real-time RT-PCR
- Expression-array and expression-chip analysis cDNA synthesis
- RNase and S1 nuclease protection
- Northern-, dot-, and slot-blot analysis
Supporting data and figures
High-quality RNA from different donors.
Blood was collected from 12 donors into PAXgene Blood RNA Tubes. RNA was purified using the QIAsymphony PAXgene Blood RNA Kit on the QIAsymphony SP. RNA was analyzed with an Agilent 2100 Bioanalyzer. Clear enrichment of small RNA species is shown in the gel-like image by the corresponding peaks in the donor RNA next to the marker band.
Specifications
| Features | Specifications |
|---|---|
| applications | Quantitative, real-time RT-PCR, expression array and chip analysis, next-generation sequencing, cDNA synthesis, RNase and S1 nuclease protection, northern, dot, and slot blot analysis, primer extension |
| timeperrun | 72 samples in 6 h 15 min |
| processing | Automated on the QIAsymphony |
| format | Magnetic beads |
| sampleamount | 2.5 ml |
| yield | 8–16 µg |
| mainsampletype | Whole blood |
| elutionvolume | 80 µl or 120 µl or 200 µl |
| technology | Magnetic particles technology |
Resources
Brochures & Guides en (2)
Safety Data Sheets (1)
Labware Files and Documents (1)
Kit Handbooks (1)
Application/Protocol Documents (1)
Application Notes (1)
Scientific Posters (1)
Certificates of Analysis (1)