用于质粒 DNA 提取的 QIAGEN Plasmid Kit

纯化至多10 mg转染级质粒或柯斯质粒DNA

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QIAGEN Plasmid Mini Kit (25)

目录编号 / ID. 12123

25 QIAGEN-tip 20, Reagents, Buffers

￦309,000.00

KitBufferColumnCartridge

Plasmid Kit

Plasmid Buffer Set

QIAGEN-tip

QIAfilter Cartridge

离心柱类型

Mini

Midi

Maxi

Mega

Giga

制备

100

QIAGEN Plasmid Mini Kit (25) 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

QIAGEN Plasmid Kits 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

纯化得到相当于连续2次CsCl梯度离心得到的DNA纯度
高产量质粒DNA
经济的制备方法
使用LyseBlue，获得最佳的裂解效果和最大的DNA产量

产品详情

QIAGEN Plasmid Kits应用重力流阴离子交换柱纯化转染级质粒DNA。该试剂盒可纯化获得至多10 mg的质粒DNA。离心获得澄清的裂解产物和异丙醇沉淀。

绩效

QIAGEN Plasmid Kit利用重力流QIAGEN阴离子交换柱技术高效纯化质粒DNA。产量可达10 mg（Giga）、2.5 mg（Mega）、500 µg（Maxi）、或100 µg（Midi）高拷贝质粒DNA（培养体积取决于质粒拷贝数、插入片段大小、宿主菌株和培养基。）QIAGEN Plasmid Kits纯化的质粒DNA适合多种应用，如：转染（参见"Transfection efficiency versus plasmid purification method"）、克隆和体外转录。

原理

QIAGEN-tips内独特的阴离子交换树脂专为纯化核酸设计。它独特的分离特性，使纯化的DNA的纯度相当于连续2次CsCl梯度离心得到的DNA纯度。预装的QIAGEN-tips利用重力流原理工作，缩短了纯化质粒的手动操作时间。整套QIAGEN质粒纯化体系避免苯酚、氯仿、溴化乙锭和CsCl等有毒物质，减小对使用者和环境的危害。

程序

使用QIAGEN Plasmid Kits，细菌裂解物通过离心澄清。澄清后的裂解物上样到阴离子交换柱，质粒DNA在适当的低盐条件和pH条件下与树脂进行选择性结合。RNA、蛋白质、代谢产物和和其它低分子量杂质用中盐洗涤液去除，用高盐缓冲液洗脱纯质粒DNA（参见"QIAGEN Plasmid Kit procedures"）。得到的DNA用异丙醇沉淀浓缩和脱盐后，离心收集。

应用

QIAGEN Plasmid Kits纯化质粒DNA适合多种应用，如：

转染
克隆
PCR
体外转录

Example 1
	12123	12125	12143	12145	12145X4	12162	12163	12165
Product	Plasmit Kit	Plasmit Kit	Plasmit Kit	Plasmit Kit	Plasmit Kit	Plasmit Kit	Plasmit Kit	Plasmit Kit
Product Type	Kit	Kit	Kit	Kit	Kit	Kit	Kit	Kit
Column Type	Mini	Mini	MIDI	MIDI	MIDI	MAXI	MAXI	MAXI
Preparations	25	100	25	100	400	10	25	100

Example 2
CatNo	Product	Product Type	Column Type	Preparations
12123	Plasmit Kit	Kit	Mini	25
12125	Plasmit Kit	Kit	Mini	100
12143	Plasmit Kit	Kit	MIDI	25
12145	Plasmit Kit	Kit	MIDI	100
12145X4	Plasmit Kit	Kit	MIDI	400
12162	Plasmit Kit	Kit	MAXI	10
12163	Plasmit Kit	Kit	MAXI	25
12165	Plasmit Kit	Kit	MAXI	100

辅助数据和图表

Transfection efficiency versus plasmid purification method.

Different pRSVcat DNA preparations using the methods indicated were introduced into the indicated cell lines by liposome-mediated transfection, and the efficiencies determined by measuring CAT expression levels after 40 h. Each bar represents the mean of 4 independent transfections (2 transfections with each of 2 independent plasmid preparations). The highest transfection efficiency was achieved with QIAGEN Plasmid Kits.

规格

特点	规格
technology	Anion-exchange technology
culturevolumestartingmaterial	2.5–5 liters culture volume
yield	<10 mg
processing	Manual (centrifugation)
samplesperrunthroughput	1 sample per run
timeperrunorprepperrun	320 min
applications	Transfection, cloning, sequencing, capillary sequencing etc.
plasmidtype	High-copy, low-copy, cosmid DNA

资源

April 2012

This protocol is designed for the rapid, easy, and non-toxic preparation of up to 2 mg genomic DNA from not more than 2 g of tissue using QIAGEN-tip 2500. QIAGEN® Genomic-tips 20/G, 100/G, and 500/G can also be used with this protocol by reducing the amount of starting material according to the table on page 2. The purified genomic DNA ranges in size from 50-150 kb.

This protocol is designed for isolation of up to 200 μg RNA from 150 mg plant tissue or up to 1 mg RNA from 600 mg plant tissue and is for use with QIAGEN-tip 100 or QIAGEN-tip 500, respectively.

This protocol is for purification of up to 100 µg endotoxin-free plasmid DNA using QIAGEN-tip 100.

This protocol is designed to provide up to 150 μg BAC/PAC/P1 DNA or up to 400 μg cosmid DNA.

Endotoxin-free DNA is essential for gene therapy research and will improve transfection into sensitive eukaryotic cells.

The procedure has been used successfully for isolation of different medium-copy-number plasmids carrying pHM1519 or pBL1 origins of replication from Corynebacterium glutamicum ATCC 13032. Yield of plasmid DNA was typically 0.4-1.5 µg per ml LB culture, although yield was dependent on the vector, the insert, and the size of the plasmid.

The procedure has been used successfully for isolation of high- and low-copy-number plasmids from various Bacillus subtilis strains. Yield of plasmid DNA was typically 10-20 µg plasmid DNA from 100 ml culture.

The procedure has been used successfully for isolation of 110 kb P1 DNA (pAdsacBII with an 80 kb insert) from Escherichia coli strain NS3529. Yield of P1 DNA was typically 10-50 µg from 500 ml culture.

The procedure has been used successfully for isolation of a variety of medium-copy-number shuttle vectors from S. xylosus, S. carnosus, S. epidermidis, and S. aureus. Yield of plasmid DNA was typically 2-10 µg from 50 ml culture.

The procedure has been used successfully for isolation of cryptic plasmids (pLC2-based) from mesophilic Lactobacillus strains such as L. sake and L. curvatus. Yield of plasmid DNA was typically 10-20 µg plasmid DNA from 100 ml culture.

This procedure has been used successfully for isolation of 150-250 kb BAC DNA from a mouse-BAC library cloned in pBeloBAC11 from Escherichia coli strain HB101/r. The yield of BAC DNA from 100 ml culture was typically 20-40 μg.

The procedure has been used successfully for isolation of high-copy-number plasmids from Proteus vulgaris and Proteus mirabilis. Yield of plasmid DNA was typically 3-8 µg DNA per ml culture.

The procedure has been used successfully for isolation of plasmids SCP2 and SCP2* as well as the plasmids listed in Table 1 (see next page) from S. coelciolor and S. lividans strains.

The procedure has been used successfully for isolation of the large (128 kb), very-low-copynumber (1-2 copies per cell) plasmid pHCG3 and its derivatives from Oligotropha carboxidovorans. Yield of plasmid DNA was typically 3-6 µg plasmid DNA from 200 ml culture.

The procedure has been used successfully for isolation of linear plasmids from Borrelia burgdorferi sensu lato species, which include Borrelia burgdorferi sensu stricto, Borrelia afzelli, and Borrelia garinii.

The procedure has been used successfully for isolation of high-copy-number plasmids from Citrobacter freundii. Yield of plasmid DNA was typically 3-8 µg DNA per ml culture.

Download Safety Data Sheets for QIAGEN product components.

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