RT² lncRNA PCR Arrays

RT² lncRNA PCR Arrays combine real-time PCR performance and a panel of long noncoding RNAs related to a disease state or biological pathway to deliver sensitive, specific lncRNA profiling. These arrays are especially suited for researchers who prefer RT-PCR technology and require the capacity to study the expression of multiple lncRNAs at once.

RT² lncRNA PCR Arrays are panels of RT² lncRNA qPCR Assays for long noncoding RNAs related by pathway or disease. Assays are designed using a computer algorithm which has been developed using an in vitro assay to ensure that the resulting primer sequences generate a single PCR product of the predicted size without primer-dimers or off-target amplification in 33 cycles of PCR amplification. The assay also ensures that the amplification efficiency of the primers is 100 +/– 10%. As a result, the algorithm designs highly effective primer sequences for SYBR Green based real-time PCR detection.
For optimal performance, RT² lncRNA PCR Arrays should be used together with the RT² First Strand Kit for cDNA synthesis and RT² SYBR Green Mastermixes for qPCR. These reagents have been formulated and pretested together with RT² lncRNA PCR Arrays. The RT² First Strand Kit includes a proprietary genomic DNA elimination step to remove any residual contamination in RNA samples before reverse transcription, thereby eliminating false positive signals. In addition, an artificial RNA control template is also included which can be used to follow the reverse transcription efficiency across samples and monitor against RNase contamination. Each of the real-time instrument-specific RT² SYBR Green Mastermixes contains SYBR Green and an appropriate reference dye to match the instrumentation available in your laboratory. RT² SYBR Green Mastermixes are available for all real-time PCR instruments from QIAGEN, Applied Biosystems, Bio-Rad, Stratagene, Eppendorf, Roche, and other major suppliers.

Simply mix the cDNA template with the appropriate ready-to-use PCR mastermix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program. RT² lncRNA PCR Arrays are compatible with all QIAGEN, ABI, Bio-Rad, Eppendorf, Roche, and Stratagene instruments.
Flexible layout and controls
RT² lncRNA PCR Arrays are available in 96-well plate, 384-well plate, and 100-well disc formats, and are used to monitor the expression of 84 lncRNA genes related to a disease state or pathway, plus 5 reference genes. Each RT² lncRNA PCR Array also includes control elements for:

• Data normalization
• Genomic DNA contamination detection
• RNA sample quality
• General PCR performance

Easy-to-use data analysis
Data can be analyzed using an easy-to-use Excel-based data analysis template or Web-based software. Data analysis is based on the ΔΔCq method with normalization of the raw data to either housekeeping genes.

RT² lncRNA PCR Arrays are highly suited for biomarker studies and other studies that require simultaneous, sensitive analysis of lncRNA expression.