TissueRuptor

• 각각의 샘플마다 새로운 probe를 사용함으로써 cross-contamination제거되는 일회용 probe 사용
• 빠르고 효과적인 파쇄 — 다양한 sample type에서의 안정적인 파쇄
• 시간 절감 효과 — 각각의 sample을 파쇄한 후, 일회용 probe는 버림
• 최적화된 protocols — 검증된 QIAGEN 정제기술 통합
• Flexibility — 여러가지 샘플 형태, lysis buffer, 파쇄용기를 자유롭게 사용가능

Genotyping, gene expression, and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA, and protein. The TissueRuptor system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table).

The TissueRuptor is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA, and protein. Optimized protocols integrate sample disruption with biomolecule purification, enabling a streamlined, efficient workflow. In addition, a range of automated solutions allow purification and analysis of biomolecules at low to high throughputs.

QIAGEN also provides RNAlater RNA Stabilization Reagent (to stabilize RNA) and Allprotect Tissue Reagent (to stabilize DNA, RNA, and protein). Tissues collected in these reagents can also be easily disrupted with the TissueRuptor.

The TissueRuptor is a portable device with a TissueRuptor Disposable Probe. The blade of the probe rotates at a high speed, causing simultaneous disruption and homogenization of a sample through a combination of turbulence and mechanical shearing. Samples that can be processed include human, animal, and plant tissues (for plant tissues that are more difficult to disrupt, the TissueLyser II is recommended). Disruption at full speed for as little as 30 seconds is usually sufficient to release nucleic acids or proteins from starting material.

After disruption of a sample, the TissueRuptor Disposable Probe can be discarded, and a new probe can be used to disrupt the next sample. Time is saved and cross-contamination is avoided, as there is no need to clean and reuse the same probe. In addition, the TissueRuptor Disposable Probe is transparent, which allows visual monitoring of the sample disruption process.

The TissueRuptor enables efficient disruption and homogenization of human, plant, and animal samples, including clotted blood. Purification of RNA, DNA, total nucleic acids, or protein can then be performed using QIAGEN kits.