For use in 2-step and 1-step qRT-PCR with SYBR® Green detection for gene expression analysis
GeneGlobe에서 구성
관심 있는 생물학적 표적을 연구하는 데 적합한 표적별 분석 및 패널을 찾거나 맞춤 설계를 진행합니다.
QuantiTect Primer Assay (200)
카탈로그 번호 / ID. 249900
For 200 x 50 µl reactions or 400 x 25 µl reactions: 10x QuantiTect Primer Assay (lyophilized) supplied in single tube
로그인 계정 가격 보기.
QuantiTect Primer Assays은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품들은 질병의 진단, 예방, 또는 치료 목적으로 사용할 수 없습니다.
GeneGlobe에서 구성
관심 있는 생물학적 표적을 연구하는 데 적합한 표적별 분석 및 패널을 찾거나 맞춤 설계를 진행합니다.
특징
- Guaranteed results with genomewide, predesigned primer pairs
- 100% PCR 효율 보장, 정확한 상대 정량
- High sensitivity and specificity
- Accurate quantification over a wide linear range
- SYBR Green detection으로 시간과 비용 절감
제품 세부 정보
QuantiTect Primer Assays are genomewide, bioinformatically validated primer sets for use in SYBR Green-based real-time RT-PCR on any cycler. Assays are available for all genes from human, rat, mouse, and many other species. Each assay for a specific gene is supplied as a lyophilized mix of forward and reverse primers that can be easily reconstituted to obtain a 10x assay solution (reaction components for real-time RT-PCR need to be ordered separately). When used in combination with QuantiFast, QuantiTect, Rotor-Gene, or FastLane Kits for SYBR Green detection, QuantiTect Primer Assays guarantee highly specific and sensitive results in real-time RT-PCR that are comparable to probe-based detection.
성능
QuantiTect Primer Assays enable reproducible quantification of RNA transcripts over a range of template amounts (see part [A] of figure " Reproducible real-time RT-PCR"). Accurate quantification is guaranteed when the assays are run using QuantiFast SYBR Green Kits or other SYBR Green kits from QIAGEN. The kits prevent the formation of PCR artifacts (see part [B] of figure " Reproducible real-time RT-PCR").
When used with QuantiFast, QuantiTect, Rotor-Gene, or FastLane SYBR Green Kits (master mixes for two-step or one-step RT-PCR), the assays may even give lower CT values compared with probe-based assays (see figure " Superior sensitivity in real-time RT-PCR"). This is due to the high PCR specificity of the kits and multiple binding of SYBR Green dye to each PCR product. Highly sensitive quantification is possible even with low-abundance transcripts.
그림 참조
원리
QuantiTect Primer Assays eliminate the need to develop and validate real-time RT-PCR assays (see figure " Time savings in development of real-time RT-PCR assays"). They can be used in both one-step and two-step qRT-PCR. Simply visit QIAGEN's GeneGlobe Web portal, and select and order an assay for any gene from human, rat, mouse, Drosophila, Arabidopsis, and other species. QuantiTect Primer Assays provide guaranteed results when used with QuantiFast, QuantiTect, Rotor-Gene, or FastLane SYBR Green Kits (master mixes for two-step or one-step RT-PCR).
그림 참조
절차
Each QuantiTect Primer Assay is supplied as a lyophilized mix of forward and reverse primers for a specific gene. The primers are reconstituted in TE, pH 8.0 to give a 10x primer solution, which is then added to the master mix supplied with a QuantiFast, QuantiTect, Rotor-Gene, or FastLane Kit for SYBR Green detection. The master mix with primers is aliquoted into PCR tubes or wells, and the individual template samples are then added.
QuantiTect Primer Assays provide guaranteed results in gene expression analysis when used in combination with one of the following kits shown in the table.
| Two-step qRT-PCR | One-step qRT-PCR |
|---|---|
| QuantiFast SYBR Green PCR Kit | QuantiFast SYBR Green RT-PCR Kit |
| Rotor-Gene SYBR Green PCR Kit | Rotor-Gene SYBR Green RT-PCR Kit |
| QuantiTect SYBR Green PCR Kit | QuantiTect SYBR Green RT-PCR Kit |
QuantiTect Primer Assays are designed to detect RNA only where possible. For assays where detection of genomic DNA cannot be avoided, cDNA can be prepared using the QuantiTect Reverse Transcription Kit or the FastLane Cell cDNA Kit. Both kits provide cDNA synthesis with integrated removal of genomic DNA contamination.
Naming
Each QuantiTect Primer Assay available for a particular gene is given a number that appears in the product name after the gene symbol. Read more in the GeneGlobe FAQs.
응용 분야
QuantiTect Primer Assays are for gene expression analysis using one-step and two-step qRT-PCR with SYBR Green detection. They are highly suited for applications such as:
- Validation of siRNA-mediated gene knockdown
- Validation of microarray results
- High-throughput screening
지원되는 데이터 및 수치
Superior sensitivity in real-time RT-PCR.
MAPK1 (a protein kinase) and IL6 (a cytokine) in human leukocyte cDNA (1 ng) were quantified in duplicate using [A] QuantiTect Primer Assays and the QuantiTect SYBR® Green PCR Kit, or [B] probe-based assays and a real-time PCR kit from Supplier AII. QuantiTect Primer Assays provided greater sensitivity than the probe-based assays, as demonstrated by the lower CT values, as well as high specificity, as indicated by the single peaks in melting curve analysis.
사양
| 특징 | 사양 |
|---|---|
| applications | Gene expression analysis |
| kitforthisapplicationontheinstrument | QuantiFast SYBR Green PCR or RT-PCR Kit, QuantiTect SYBR Green PCR or RT-PCR Kit |
| detection | SYBR Green I |
| realtimeorendpoint | Real-time |
| species | Human, mouse, rat, drosophila arabidopsis, chicken, dog, nematode, and zebrafish |
| format | Lyophylized primer set |
리소스
Brochures & Guides en (1)
Kit Handbooks (1)
Articles (1)
Safety Data Sheets (1)
Scientific Posters (2)
Gene Expression Analysis (2)
Certificates of Analysis (1)