QIAcuity Probe PCR Kit

供与 QIAcuity 数字 PCR 仪器一起使用

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

QIAcuity Probe PCR Kit (5 ml)

目录编号 / ID. 250102

5 x 1 ml 4x 浓缩 QIAcuity Probe Mastermix，8 x 1.9 ml 水

登录要查看您的账户定价。

体积

5 ml

1 ml

25 ml

QIAcuity Probe PCR Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

适用于单重和最多 5 重反应
4x 浓缩预混液，可加载更多样本
针对在 QIAcuity Nanoplate 中的微流体使用优化
更少的非特异性 PCR 扩增
符合 REACH 法规

产品详情

QIAcuity Probe PCR Kit 包含一份用在 QIAcuity Nanoplate 微流体中优化的 4x 浓缩即用型预混液。该试剂盒提高了基于探针法的数字 PCR 的特异性和效率，可提供准确的单重或最多 5 重的定量分析结果。QIAcuity 探针法预混液经过优化，提高特异性，从而可在 QIAcuity dPCR 仪器上准确定量 gDNA 或 cDNA。

该试剂盒应与 QIAcuity Digital PCR System 和 QIAcuity Nanoplate结合使用。

您想要进一步了解该产品并让我们的 dPCR 专家联系您吗？请在这里登记，我们将很快与您联系。

绩效

卓越的性能
基于水解探针法的 dPCR， QIAcuity 预混液中使用了最新版的 QIAGEN 高品质 DNA 聚合酶。QIAGEN 的专有技术与经过良好验证的专门针对纳米微孔板微流体优化的缓冲液技术及新的 QuantiNova DNA 聚合酶的独特结合，可在灵敏度、重复性及效率等方面获取高度一致的结果。

使用 QIAcuity Probe PCR Kit 可进行基于探针法的单重到五重 dPCR检测
QIAcuity Probe PCR Kit 中的独特预混液可让您在 QIAcuity Nanoplate 单孔中对最多 5 个丰度大为不同的靶标基因进行准确定量。这可节省时间和金钱，降低所需的样本材料用量。此外，双重或多重 PCR获取的数据与单重 PCR 中获得的数据具有一致性。

反应稳定性可长达 100 个小时
QIAcuity PCR 混合物可在 30°C 下存放长达 100 个小时，而不会影响后续反应的性能。优异的稳定性——即便在室温不使用冷却剂的情况下，长时间存放后仍保持性能的稳定性，使得 QIAcuity Probe PCR Kit 成为高通量反应体系构建及孔板堆叠处理时的理想选择。

原理

QIAcuity Probe PCR Kit 采用新型抗体介导的热启动机制，可确保 cDNA 或 gDNA 单重或多重分析时的高特异性。在低温下，QuantiNova DNA 聚合酶在 QuantiNova 抗体和 QuantiNova Guard（一款具有复合物稳定化作用的新型添加剂）的作用下保持在失活状态。这提高了热启动的严格性并防止非特异性退火引物的延伸及引物二聚体的形成。在温度升高到 95°C 的两分钟内，QuantiNova 抗体和 QuantiNova Guard 将发生变性，QuantiNova DNA 聚合酶将被激活，从而启动 PCR 扩增。

有关纳米微孔板中 dPCR 反应的原理说明，请参见此处。

程序

与 qPCR 实验一样，样本制备包括将预混液、探针和引物转移到一个 96 孔或 24 孔纳米微孔板中，随后添加样本。该系统将微分化、热循环和成像集成到单一全自动化的仪器上，使用户可在 2 小时内获得结果。您可在 Suite Software 上执行分析，该软件可针对您的靶序列及质量控制（例如阳性样本或无模板对照 [NTC]）给出以每微升拷贝数表示的浓度。该分析还可在同一局域网 (LAN) 上的远程计算机上执行。

应用

QIAcuity Probe PCR Kit 与 QIAcuity Digital PCR System 和 QIAcuity Nanoplate 的结合使用可让您在各种应用中进行 cDNA 靶标和 gDNA 定量分析，这些应用包括：

罕见突变检测
拷贝数变异分析
基因表达分析
病原体检测
基因分型
miRNA 研究
细胞和基因治疗
宿主细胞 DNA 残留定量
废水监测

辅助数据和图表

简单快速的基于纳米微孔板的工作流程

微分化、热循环和成像均集成到单一全自动的仪器上，可在 2 小时内获取实验结果。纳米微孔板格式支持在反应体系配制中的前端自动化操作，因而可进一步减少手动操作时间。

资源

For Version 2.0

For Version 2

Version 4.0

The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.

After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.

Required QIAcuity Software Suite version: Version 1.2 or higher.

Version 2.1

Version 1.2

The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

The following browsers are supported in the QIAcuity Software Suite:

-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)

The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.

Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.

The new improvements are as follows:

-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting

For Version 1.2

For Version 1.0

Fast. Scalable. Reliable.

For use with QIAcuity systems

July 2021

The goal of this work was to compare performance of quantitative PCR (qPCR) and digital PCR (dPCR) in the quantification of gene expression and Wolbachia abundances in Nasonia parasitoid wasps.

This study tested a workflow for quantitation and qualification of AAV samples using a duplex assay on the QIAcuity dPCR instrument targeting both an insert (GFP) and the viral backbone (AAV2-ITR). With very low intra-assay and inter-assay CVs <6.5%, we demonstrate one of the main benefits of dPCR: reproducibility.

Here we provide an integrated rAAV genome titer method using the QIAcuity Digital PCR (dPCR) System with detailed parameters for high assay performance. Using this optimized method for pre-PCR handling of in-process rAAV samples, the results demonstrated that QIAcuity dPCR system generates the same level of accuracy and precision as the current gold standard ddPCR system but with much faster sample-to-result times (2 hours vs 7 hours) and higher overall throughput and scalability.

Limitations of conventional PCR and qPCR when dealing with difficult, low-volume samples and complex mixtures with high background of competitive molecules and inhibitors have posed frequent challenges for researchers and clinicians in their routine work. With the new generation of PCR technologies, digital PCR has opened doors for diverse applications, and researchers are learning to ask questions only digital PCR can answer. Join QIAGEN's webinar on how digital PCR can help take your research applications through and beyond those challenges.

This presentation will introduce dPCR, discuss its advantages, and outline how the approach might be used to improve measurement in areas like clinical diagnosis, alone or in conjunction with other methods.

In this expert webinar, Dr. Kubista will share with you the experience he and his team have gathered at the TATAA Biocenter, developing applications and providing services using digital PCR for nearly 12 years. They have experienced all the problems common to dPCR analytical workflows and developed robust standard operating procedures to minimize the risk of error and maximize robustness and repeatability, and developed various controls to test the performance and validate the methods. He will also discuss dPCR assay design and validation and then focus on strategies for copy number determination and rare mutation detection.

The QIAGEN digital PCR technology and its expanded capability will not only transform the portfolio of conventional qPCR applications but also provide a more rapid, accurate, and sensitive method for finding answers to difficult biological questions.

As the digital PCR technology evolves and becomes more accessible and affordable, the transition from qPCR and adoption of dPCR will hopefully no longer remain a challenge. Experts share insights in an upcoming webinar about the fully integrated, rapid, and highly flexible digital PCR portfolio from QIAGEN.

The goal of this work was to compare performance of quantitative PCR (qPCR) and digital PCR (dPCR) in the quantification of gene expression and Wolbachia abundances in Nasonia parasitoid wasps.

Fast. Scalable. Reliable.

Version 1.0

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.

This new version of the control software includes system stability and performance reliability improvements. We highly recommend to update the control software of your QIAcuity instrument.

Note: The installation requires approximately 90 minutes.

Version 2.0

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis

Besides system stability and performance reliability improvements, this new version of the control software offers advanced user management and audit trail functionalities. Together with the QIAcuity Software Suite 2.0, the QIAcuity systems now support 21 CFR Part 11 regulations for users working under GMP.

Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.

Note: Upgrading the QIAcuity Control Software to version 2.0 will require upgrading the QIAcuity Software Suite to version 2.0.

Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.0. It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!

Version 2.1

Beside of improvements and bug fixes, the new features mainly address the GMP requirements by offering additional support for 21 CFR part 11 compliance and get the software ready for future Nanoplate support.

For more information, please refer to the Release Note: QIAcuity Instrument Control Software, Version 2.1.

SHA1 checksum: 0DD41A8DE46D10E81F538ACB71AC184170D39FE0

Version 1.1.3

Version 1.2

-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)

Version 2.0

The QIAcuity Software Suite 2.0 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

This new software version now supports 21 CFR Part 11 regulations for users working under GMP. This includes the following:

-Advanced User Management
-Audit Trail
-Signatures

Additional changes compared with the previous software version include the following:

-Usability improvements
-Improved dust detection
-Hyperwell expansion to CNV and GEX analysis
-CSV export functionality of multiple positive partitions for linkage analysis, drop-off assays, etc.
-Raw data export

Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.

Important: A direct update of the Software Suite from version 1.1.3 to 2.0.20 is not possible. Please follow the instructions in the User Manual on how to update to version 1.2.18 first before updating the Software Suite to version 2.0.20.

Before you start upgrading to version 1.1.3, please make sure that all plates are imported and visible in the plate overview of the QIAcuity Software Suite.

Caution: Not following the instructions may result in a loss of your previous plate data!

Please contact QIAGEN Technical Services if you are unsure and require technical support.

SHA1 checksum: C60C4EEC349DF1C037796016D32A93EEC19B54A0

Version 2.1

Beside of usability improvements and bug fixes, the new features address GMP requirements by offering additional support for 21 CFR part 11 compliance, copy number variation (CNV) analysis, support of future Nanoplates, and improved 1D scatterplot views.

For more information, please refer to the Release Note: QIAcuity Software Suite, Version 2.1

SHA1 checksum: 6D51E273B26FA7D7E0EDA843CB3DCD74B38FA5B1

For Version 1.0

For Version 1.2

For Version 2.0

For Version 2

Version 2.1

For use with QIAcuity systems

July 2021

TB Management

Transplant

Infectious Disease

Oncology

Sexual & Reproductive Health

Sample Processing

Solutions for Laboratory-Developed Tests

QIAcuity Probe PCR Kit

供与 QIAcuity 数字 PCR 仪器一起使用

QIAcuity Probe PCR Kit (5 ml)

特点

产品详情

绩效

原理

程序

应用

辅助数据和图表

简单快速的基于纳米微孔板的工作流程

资源