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RNeasy MinElute Cleanup Kit (50)
Cat no. / ID. 74204
50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
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The RNeasy MinElute Cleanup Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Product Details
The RNeasy MinElute Cleanup Kit enables cleanup and concentration of RNA from enzymatic reactions or other samples using specialized RNeasy MinElute spin columns based on silica-membrane technology. The kit can also be used to desalt RNA samples. Up to 45 µg RNA can be purified in a volume as low as 10 µl. Purification can be fully automated on the QIAcube Connect.
Performance
The RNeasy MinElute Cleanup Kit provides high-quality total RNA, free from impurities or enzymatic inhibitors, with A260/A280 ratios of 1.9–2.1 (see figure " High-quality RNA"). RNA amounts corresponding to less than one cell (as little as 1 pg) can be concentrated (see figure " Concentration of RNA"). A large amount of RNA (up to 45 µg) can be purified and is suitable for use in sensitive assays (see figure " Reliable cleanup of RNA"). Reaction volumes can be kept small in downstream applications, giving increased reaction efficiency. The high purity RNA allows more of the sample to be used in reactions without inhibition (see figure " Efficient removal of real-time RT-PCR inhibitors").
See figures
Principle
The RNeasy MinElute Cleanup Kit is designed to purify and concentrate RNA from enzymatic reactions (e.g., labeling, in vitro transcription), RNA isolated by alcohol-precipitation and organic-extraction methods, as well as to desalt RNA samples, and concentrate RNA prepared by silica-membrane procedures (e.g., PAXgene Blood RNA preps). RNeasy technology simplifies total RNA isolation by combining guanidine-isothiocyanate lysis with silica-membrane purification.
Procedure
Guanidine-isothiocyanate–containing lysis buffer and ethanol are added to the sample to create conditions that promote selective binding of RNA to the RNeasy MinElute membrane. The sample is then applied to the RNeasy MinElute spin column. RNA binds to the silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in water.
Enzymatic reactions or crude RNA preps are simultaneously cleaned up and concentrated in less than 15 minutes. In comparison, time-consuming concentration and cleanup by alcohol precipitation can result in loss of RNA, especially from small samples. The RNeasy MinElute procedure is faster than vacuum centrifugation, which only concentrates the sample without removing salts and other impurities. The unique design of RNeasy MinElute spin columns enables concentration of purified RNA to as little as 10 µl for downstream applications such as microarray analysis and real-time RT-PCR. Purification can be fully automated on the QIAcube Connect.
Applications
RNA purified with RNeasy MinElute technology is high-quality and ideal for use in all applications including:
- Northern, dot, and slot blotting
- End-point RT-PCR
- Quantitative, real-time RT-PCR
- Array analysis
- Poly A+ RNA selection
Supporting data and figures
High-quality RNA.
Total RNA was isolated from HeLa cells using an acid-phenol method plus cleanup and concentration using the RNeasy MinElute Cleanup Kit. The high quality of the RNA is shown by scanning with the Agilent 2100 Bioanalyzer.
Specifications
| Features | Specifications |
|---|---|
| applications | PCR, qPCR, real-time PCR, microarray |
| format | MinElute Spin column |
| sampleamount | 200 µl |
| elutionvolume | 10–14 µl |
| processing | Manual |
| mainsampletype | (Crude) RNA preps |
| purificationoftotalrnamirnapolyamrnadnaorprotein | RNA |
| timeperrunorperprep | <15 minutes |
| technology | Silica technology |
| yield | 45 µg |
Resources
Kit Handbooks (1)
Safety Data Sheets (1)
Brochures & Guides en (1)
Supplementary Protocols (2)
Quick-Start Protocols (1)
User-Developed Protocols (1)
Gene Expression Analysis (1)
Certificates of Analysis (1)
Publications
Silencing of stathmin induces tumor-suppressor function in breast cancer cell lines harboring mutant p53.
Oncogene; 2006; 26 (7):1003-12 2006 Aug 14 PMID:16909102
The extracellular nucleotide UTP is a potent inducer of hematopoietic stem cell migration.
Blood; 2006; 109 (2):533-42 2006 Sep 28 PMID:17008551
The lspA gene, encoding the type II signal peptidase of Rickettsia typhi: transcriptional and functional analysis.
J Bacteriol; 2006; 189 (2):336-41 2006 Nov 10 PMID:17098907
Selection and cloning of poly(rC)-binding protein 2 and Raf kinase inhibitor protein RNA activators of 2',5'-oligoadenylate synthetase from prostate cancer cells.
Nucleic Acids Res; 2006; 34 (22):6684-95 2006 Dec 1 PMID:17145707
Additional freeze hardiness in wheat acquired by exposure to -3 degreesC is associated with extensive physiological, morphological, and molecular changes.
J Exp Bot; 2006; 57 (14):3601-18 2006 Sep 12 PMID:16968883