QIAprep 96 Turbo Miniprep Kit

每孔纯化至多20 µg分子生物学级纯度的质粒DNA

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QIAprep 96 Turbo Miniprep Kit (4)

目录编号 / ID.   27191

For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 and QIAprep 96 Plates; S-Blocks, reagents, buffers, collection microtubes (1.2 ml), caps
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Kit
QIAprep 96 Turbo Kit
QIAprep 96 Turbo Core Kit
用于
QIAvac 96
BioRobot Universal
制备
4 x 96
24 x 96
QIAprep 96 Turbo Miniprep Kit 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 高效的纯化流程
  • 45分钟内获得即用型质粒DNA
  • 持续获得分子生物学级纯质粒DNA

产品详情

QIAprep 96 Tube Miniprep Kit提供QIAprep 96和TurboFilter 96孔板,每孔可自动纯化获得至多20 μg分子生物学级纯质粒DNA。

绩效

QIAprep 96 Turbo Miniprep Kit可纯化获得多达20 µg纯质粒或科斯质粒DNA,得到的产物适用于多种常规分子生物学级纯度的应用,如测序和克隆(参见"High-quality DNA for automated sequencing")。

除了从Escherichia coli纯化得到质粒,QIAprep Kits还可以从Saccharomyces cerevisiaeBacillus subtilisAgrobacterium tumefaciens纯化得到质粒DNA。请联系QIAGEN技术专家或当地经销商获得相关应用的实验方案。

原理

QIAprep 96孔板(参见"QIAvac 96 with 96-well plate")含独特的硅胶膜,在高浓度离液盐中可结合多达20 µg DNA,然后用少量低盐缓冲液洗脱。QIAprep膜技术去除了耗时的苯酚−氯仿抽提和乙醇沉淀,解决了树脂松散和悬浮液带来的问题与不便。从QIAprep 96孔板洗脱得到的质粒DNA可直接使用,无需沉淀、浓缩或脱盐。

TurboFilter 96孔板专用于快速澄清在高浓度离液盐条件下形成的细菌裂解液。粗细菌裂解物通过TurboFilter 96孔板真空滤除,无需离心处理。无颗粒裂解液直接流入QIAprep 96孔板中,即用于纯化和洗脱。TurboFilte技术简化了QIAprep纯化质粒的操作流程。

程序

QIAprep 96 Turbo Miniprep Kits纯化质粒只需简单的结合−洗涤−洗脱过程(参见"The QIAprep procedure")。首先,裂解细菌培养基,裂解液通过TurboFilter过滤。澄清后的裂解液直接流入QIAprep 96孔板中,质粒DNA与硅胶膜结合。洗去杂质后,用少量的洗脱缓冲液或水洗脱,得到纯DNA。

QIAprep 96 Turbo整个操作流程在96孔板上,减少样本的手动操作时间,能够在45分钟内完成96个样本的平行处理。该试剂盒利用96孔板培养和裂解细菌培养基。QIAvac 96装置用于96孔板模式的过滤,因此通过TurboFilter 96 孔板澄清的裂解产物可直接流入QIAprep 96孔板中,平行获得高通量的96个样本。

应用

QIAprep 96 Turbo Miniprep Kits可重复纯化得到的纯DNA适合多种应用,包括:

  • 筛选
  • PCR 
  • 测序
  • 限制性酶切 
  • 连接和转化

资源

试剂盒操作手册 (1)
补充实验方案 (2)
This protocol is designed for 96 parallel plasmid DNA preparations from 1.3 ml overnight cultures.
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)

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