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ipsogen
PML-RARA bcr1 Kit
ipsogen
PML-RARA bcr1 Kit
采用real-time PCR对PML-RARA bcr1融合基因转录本进行定量检测
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
✓ 快速可靠的(再)订购
ipsogen
PML-RARA bcr1 Kit (24)
目录编号 / ID.
672113
For 24 reactions: ABL Control Gene Standards, PML-RARA bcr1 Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix PML-RARA bcr1 Fusion Gene
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
✓ 快速可靠的(再)订购
特点
灵敏、准确的定量检测试剂
符合EAC要求的标准化试剂,结果具有可重复性
即用型解决方案,便利的工作流程
产品详情
ipsogen
PML-RARA bcr1 Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对PML-RARA bcr1融合基因转录本在总RNA中相对于ABL对照基因的表达进行可靠、灵敏的定量检测。
绩效
为确保高灵敏度,
ipsogen
PML-RARA bcr1 Kit已经过优化,使用预稀释的质粒标准品和引物探针混合液,检测PML-RARA bcr1和ABL转录本(参见
Accurate detection of PML-RARA bcr1 fusion gene plasmid standards
和
Reliable detection of ABL plasmid standards
)。
查看图表
Accurate detection of PML-RARA bcr1 fusion gene plasmid standards.
Reliable detection of ABL plasmid standards.
PML-RARA bcr1 gene transcript.
ABL control gene transcript.
原理
ipsogen
PML-RARA bcr1 Kit为即用型试剂盒,采用real-time PCR技术,检测PML-RARA bcr1融合基因转录本。该试剂盒可扩增并检测总RNA中相对于ABL对照基因表达的特定PML-RARA bcr1融合基因转录本(参见
PML-RARA bcr1 gene transcript
和
ABL control gene transcript
)。该试剂盒灵敏度高,特异性强,可重复性好。
ipsogen
PML-RARA bcr1 Kit为PML-RARA bcr1融合基因提供5个标准品稀释液,为ABL提供3个标准品稀释液。使用标准品能够实现准确的定量检测。已与欧洲抗癌协会(EAC)联合对检测方法的标准化进行验证,
ipsogen
PML-RARA Kit正是基于这种技术。
查看图表
PML-RARA bcr1 gene transcript.
ABL control gene transcript.
Accurate detection of PML-RARA bcr1 fusion gene plasmid standards.
Reliable detection of ABL plasmid standards.
程序
第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增cDNA。
ipsogen
PML-RARA bcr1 Kit可对PML-RARA bcr1和ABL转录本进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。
应用
ipsogen
PML-RARA bcr1 Ki能够对PML-RARA bcr1转录本相对于ABL对照基因的表达进行可靠、灵敏的定量检测。仅适用于科研。
辅助数据和图表
PML-RARA bcr1 gene transcript.
PML-RARA bcr1 gene transcript.
资源
试剂盒操作手册 (1)
(EN) - ipsogen FusionQuant Handbook
EN
PDF
(937KB)
安全数据表 (1)
Safety Data Sheets
EN
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)
Certificates of Analysis
EN