QIAcuityDx Nanoplates and Accessories

For use with QIAcuityDx digital PCR instruments

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QIAcuityDx Nanoplate 26k 24-well (10)

Cat no. / ID. 260001

10 QIAcuityDx Nanoplate 26k 24-well, 11 QIAcuity Nanoplate Seals

Product TypeAccessories

QIAcuityDx Nanoplate

QIAcuity Nanoplate

Nanoplate Seals

Nanoplate Tray

Nanoplate Adapter

The QIAcuityDx Nanoplate 26k 24-well is intended for in vitro diagnostic use.

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✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

QIAcuityDx Nanoplate 26k 24-well
Majority of QIAcuity life science configurations supported in Utility mode
Up to 8500 or 26,000 partitions per well
SBS format

Product Details

Nanoplates are microfluidic digital PCR plates that enable 24 or 96 samples to be run with up to 8500 or 26,000 partitions per well. All four nanoplates are designed to run on QIAcuityDx Four instruments.

In addition, QIAcuity life science configurations across 24 or 96 reactions and varying partitions between 8.5k and 26k may be used within the Utility mode of QIAcuityDx. Note: The IVD mode only allows use of QIAcuityDx plates.

The different nanoplate configurations can only be used within the QIAcuity dPCR instrument family. In the Utility mode, use the dedicated QIAcuity Nanoplate Adapter when performing automated liquid handling and PCR setup in a QIAcuity nanoplate using the QIAgility. Once done, load the plate onto the QIAcuityDx Four instrument for the dPCR reaction.

Would you like to learn more about the product and be contacted by one of our dPCR specialists? Contact us for more information and we will get in touch with you shortly.

Use of QIAcuityDx Nanoplates and Accessories in Utility and IVD mode

Product	Cat. no.	Utility mode	IVD mode
QIAcuityDx Nanoplate 26k 24-well	260001	✓	✓
QIAcuity Nanoplate 26k 24-well	250001	✓
QIAcuity Nanoplate 8.5k 24-well	250011	✓
QIAcuity Nanoplate 8.5k 96-well	250021	✓
Nanoplate Seals*	250099	✓
Nanoplate Tray	250098	✓	✓
QIAcuity Nanoplate Adapter	9027242	✓

*Nanoplate Seals are for use with QIAcuity Nanoplates only.

Performance

These are specially designed plates used for digital PCR reactions in QIAcuityDx instruments. We offer four nanoplate types, all in the Society for Biomolecular Screening (SBS) format, but with different specifications for different application needs.

Nanoplate – features and specifications
Type	Frame color	Specifications	Applications
QIAcuityDx Nanoplate 26k 24-well (IVD mode or Utility mode)	Red	24-well x approx. 26,000 partitions 40 µL dPCR reaction per well	Rare mutation detection, liquid biopsy, pathogen detection, etc.
QIAcuity Nanoplate 26k 24-well (Utility mode)	Blue	24-well x approx. 26,000 partitions 40 µL dPCR reaction per well
QIAcuity Nanoplate 8.5k 24-well (Utility mode)	White	24-well x approx. 8500 partitions 12 µL dPCR reaction per well	Copy number variation analysis, gene expression analysis, NGS library quantification, genome edit detection, etc.
QIAcuity Nanoplate 8.5k 96-well (Utility mode)	Gray	96-well x approx. 8500 partitions 12 µL dPCR reaction per well

Principle

In just 3 simple steps, you can get your dPCR results in approximately 2 hours: pipette and load, run the experiment, analyze results. The principle of the dPCR reaction in the nanoplates is described here.

Procedure

Just like in a qPCR workflow, sample preparation includes the transfer of master mix, probes and primers to a 24- or 96-well nanoplate followed by the addition of samples. The system integrates partitioning, thermocycling and imaging into a single, semi-automated instrument that achieves sample to result in approximately 2 hours. Analysis is performed using the Software Suite. This provides the concentration in copies per microliter of your target sequence when using the Utility mode and can also be used for quality control, such as for positive samples or No Template Control (NTC). This analysis can be extended to remote computers within the same local area network (LAN).

Applications

QIAcuity Nanoplates, in combination with the QIAcuityDx Four and QIAcuityDx Universal MasterMix Kit, enable digital PCR applications, including:

Rare mutation detection
Copy number variation analysis
Gene expression analysis
Pathogen detection
Genotyping
Cell and gene therapy
Residual DNA quantification

Supporting data and figures

A simple and rapid plate-based workflow

Partitioning, thermocycling and imaging are all integrated into a single semi-automated instrument, delivering results in approximately two hours. Nanoplate formats are suitable for front-end automation for sample preparation when using the Utility mode, further reducing hands-on time.

Resources

For Version 2.0

For Version 2

Version 4.0

The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.

After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.

Required QIAcuity Software Suite version: Version 1.2 or higher.

Version 2.1

Version 1.2

The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

The following browsers are supported in the QIAcuity Software Suite:

-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)

The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.

Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.

The new improvements are as follows:

-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting

For Version 1.2

For Version 1.0

The QIAgility instrument is a liquid handler designed for automating PCR setup. For compatibility with the QIAcuity, we developed an adapter to secure up to two nanoplates onto the deck of the QIAgility. Using the QIAgility software, we have optimized a protocol that works for all nanoplate types and QIAcuity applications. Here we report the performance of a front end automated QIAgility dPCR nanoplate setup procedure for use with the QIAcuity dPCR system.

The QIAcuity digital PCR system combined with the QIAcuity One-Step Viral RT-PCR Kit enables precise detection and quantitation of vector-borne viruses in mosquitoes. The results presented in this comparison study showed that digital PCR is a powerful tool for absolute quantitation of low abundant targets and is a more reliable detection method than qPCR. Multiplexing allows detection and quantitation of multiple targets in a single reaction more efficiently by increasing sample throughput at a reduced cost per target.

Digital PCR is a superior method to qPCR for the detection and absolute quantification of low concentration target templates. There are multiple digital PCR systems on the market that differ in numerous aspects including the amount of dead volume, which is the volume that is loaded but not analyzed by the given instrument. While it has been speculated that dead volume could impact the sensitivity of dPCR applications, here we provide data to support the conclusion that the most important factors in determining the relative sensitivity of each system are template addition volume and template analyzed volume. In summary, data provided herein demonstrate that higher template addition volumes can overcome any limitations that dead volume may have on the sensitivity of a dPCR application.

The goal of this work was to compare performance of quantitative PCR (qPCR) and digital PCR (dPCR) in the quantification of gene expression and Wolbachia abundances in Nasonia parasitoid wasps.

This study tested a workflow for quantitation and qualification of AAV samples using a duplex assay on the QIAcuity dPCR instrument targeting both an insert (GFP) and the viral backbone (AAV2-ITR). With very low intra-assay and inter-assay CVs <6.5%, we demonstrate one of the main benefits of dPCR: reproducibility.

Here we report the use of saliva samples in combination with dPCR as a suitable alternative to screen for individuals infected with SARS-CoV-2.

Here we demonstrate how to optimize your assays on a microfluidic nanoplate-based digital PCR system, the QIAcuity, and provide recommendations for a seamless transfer. Moreover, the QIAcuity dPCR workflow is very similar to qPCR.

Here we compared the performance of qPCR and the nanoplate dPCR techniques. The digital PCR method on the QIAcuity significantly improved precision when measuring copy number states and sensitivity of mutation detection through absolute quantification and reduced standard error. This is advantageous in various applications, including copy number variation analysis, small fold-change and rare mutation detection.

Here we provide an integrated rAAV genome titer method using the QIAcuity Digital PCR (dPCR) System with detailed parameters for high assay performance. Using this optimized method for pre-PCR handling of in-process rAAV samples, the results demonstrated that QIAcuity dPCR system generates the same level of accuracy and precision as the current gold standard ddPCR system but with much faster sample-to-result times (2 hours vs 7 hours) and higher overall throughput and scalability.

Fast. Scalable. Reliable.

For use with QIAcuity systems

July 2021

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